6apk
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6apk is ON HOLD Authors: Lyubimov, A.Y., Mathews, I.I., Uervivojnangkoorn, M., Khosla, C., Soltis, S.M., Cohen, A.E. Description: Trans-acting tran...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Trans-acting transferase from Disorazole synthase solved by serial femtosecond XFEL crystallography== | |
| + | <StructureSection load='6apk' size='340' side='right'caption='[[6apk]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6apk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6APK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6apk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apk OCA], [https://pdbe.org/6apk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6apk RCSB], [https://www.ebi.ac.uk/pdbsum/6apk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6apk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q4U443_SORCE Q4U443_SORCE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 A using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of microcrystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed. | ||
| - | + | The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography.,Mathews II, Allison K, Robbins T, Lyubimov AY, Uervirojnangkoorn M, Brunger AT, Khosla C, DeMirci H, McPhillips SE, Hollenbeck M, Soltis M, Cohen AE Biochemistry. 2017 Sep 12;56(36):4751-4756. doi: 10.1021/acs.biochem.7b00711., Epub 2017 Aug 31. PMID:28832129<ref>PMID:28832129</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6apk" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Mathews | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Sorangium cellulosum]] | ||
| + | [[Category: Cohen AE]] | ||
| + | [[Category: Khosla C]] | ||
| + | [[Category: Lyubimov AY]] | ||
| + | [[Category: Mathews II]] | ||
| + | [[Category: Soltis SM]] | ||
| + | [[Category: Uervivojnangkoorn M]] | ||
Current revision
Trans-acting transferase from Disorazole synthase solved by serial femtosecond XFEL crystallography
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