1xxn

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[[Image:1xxn.gif|left|200px]]
 
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{{Structure
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==Crystal structure of a mesophilic xylanase A from Bacillus subtilis 1A1==
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|PDB= 1xxn |SIZE=350|CAPTION= <scene name='initialview01'>1xxn</scene>, resolution 1.70&Aring;
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<StructureSection load='1xxn' size='340' side='right'caption='[[1xxn]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene>
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<table><tr><td colspan='2'>[[1xxn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XXN FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= xynA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xxn OCA], [https://pdbe.org/1xxn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xxn RCSB], [https://www.ebi.ac.uk/pdbsum/1xxn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xxn ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xxn OCA], [http://www.ebi.ac.uk/pdbsum/1xxn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xxn RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/XYNA_BACSU XYNA_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xx/1xxn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xxn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 1.7A resolution crystal structure of recombinant family G/11 beta-1,4-xylanase (rXynA) from Bacillus subtilis 1A1 shows a jellyroll fold in which two curved beta-sheets form the active-site and substrate-binding cleft. The onset of thermal denaturation of rXynA occurs at 328 K, in excellent agreement with the optimum catalytic temperature. Molecular dynamics simulations at temperatures of 298-328 K demonstrate that below the optimum temperature the thumb loop and palm domain adopt a closed conformation. However, at 328 K these two domains separate facilitating substrate access to the active-site pocket, thereby accounting for the optimum catalytic temperature of the rXynA.
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'''Crystal structure of a mesophilic xylanase A from Bacillus subtilis 1A1'''
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Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1).,Murakami MT, Arni RK, Vieira DS, Degreve L, Ruller R, Ward RJ FEBS Lett. 2005 Nov 21;579(28):6505-10. Epub 2005 Nov 2. PMID:16289057<ref>PMID:16289057</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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1XXN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XXN OCA].
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<div class="pdbe-citations 1xxn" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Endo-1,4-beta-xylanase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Arni RK]]
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[[Category: Arni, R K.]]
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[[Category: Murakami MT]]
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[[Category: Murakami, M T.]]
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[[Category: Ruller R]]
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[[Category: Ruller, R.]]
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[[Category: Ward RJ]]
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[[Category: Ward, R J.]]
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[[Category: family 11 xylanase]]
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[[Category: thermostability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:56:01 2008''
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Current revision

Crystal structure of a mesophilic xylanase A from Bacillus subtilis 1A1

PDB ID 1xxn

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