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1xxv

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[[Image:1xxv.gif|left|200px]]
 
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{{Structure
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==Yersinia YopH (residues 163-468) binds phosphonodifluoromethyl-Phe containing hexapeptide at two sites==
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|PDB= 1xxv |SIZE=350|CAPTION= <scene name='initialview01'>1xxv</scene>, resolution 2.5&Aring;
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<StructureSection load='1xxv' size='340' side='right'caption='[[1xxv]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CLE:LEUCINE+AMIDE'>CLE</scene>, <scene name='pdbligand=FTY:DEOXY-DIFLUOROMETHELENE-PHOSPHOTYROSINE'>FTY</scene>
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<table><tr><td colspan='2'>[[1xxv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XXV FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= yopH, yop51 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 Yersinia enterocolitica])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=FTY:DEOXY-DIFLUOROMETHELENE-PHOSPHOTYROSINE'>FTY</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xxv OCA], [https://pdbe.org/1xxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xxv RCSB], [https://www.ebi.ac.uk/pdbsum/1xxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xxv ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1xxp|1XXP]], [[1qz0|1QZ0]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xxv OCA], [http://www.ebi.ac.uk/pdbsum/1xxv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xxv RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/YOPH_YEREN YOPH_YEREN] Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xx/1xxv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xxv ConSurf].
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<div style="clear:both"></div>
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'''Yersinia YopH (residues 163-468) binds phosphonodifluoromethyl-Phe containing hexapeptide at two sites'''
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==See Also==
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*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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YopH is a protein tyrosine phosphatase and an essential virulence determinant of the pathogenic bacterium Yersinia. Yersinia delivers YopH into infected host cells using a type III secretion mechanism. YopH dephosphorylates several focal adhesion proteins including p130Cas in human epithelial cells, resulting in disruption of focal adhesions and cell detachment from the extracellular matrix. How the C-terminal protein tyrosine phosphatase domain of YopH targets specific substrates such as p130Cas in the complex milieu of the host cell has not been fully elucidated. An N-terminal non-catalytic domain of YopH binds p130Cas in a phosphotyrosine-dependent manner and functions as a novel substrate-targeting site. The structure of the YopH protein tyrosine phosphatase domain bound to a model phosphopeptide substrate was solved and the resulting structure revealed a second substrate-targeting site ('site 2') within the catalytic domain. Site 2 binds to p130Cas in a phosphotyrosine-dependent manner, and co-operates with the N-terminal domain ('site 1') to promote efficient recognition of p130Cas by YopH in epithelial cells. The identification of two substrate-targeting sites in YopH that co-operate to promote epithelial cell detachment and bacterial virulence reinforces the importance of protein-protein interactions for determining protein tyrosine phosphatase specificity in vivo, and highlights the sophisticated nature of microbial pathogenicity factors.
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[[Category: Large Structures]]
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==About this Structure==
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1XXV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XXV OCA].
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==Reference==
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Two substrate-targeting sites in the Yersinia protein tyrosine phosphatase co-operate to promote bacterial virulence., Ivanov MI, Stuckey JA, Schubert HL, Saper MA, Bliska JB, Mol Microbiol. 2005 Mar;55(5):1346-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15720545 15720545]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Single protein]]
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[[Category: Yersinia enterocolitica]]
[[Category: Yersinia enterocolitica]]
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[[Category: Bliska, J B.]]
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[[Category: Bliska JB]]
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[[Category: Ivanov, M I.]]
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[[Category: Ivanov MI]]
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[[Category: Saper, M A.]]
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[[Category: Saper MA]]
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[[Category: Schubert, H L.]]
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[[Category: Schubert HL]]
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[[Category: Stuckey, J A.]]
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[[Category: Stuckey JA]]
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[[Category: catalytic domain]]
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[[Category: phosphotyrosine-binding site]]
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[[Category: substrate targeting]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:56:06 2008''
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Current revision

Yersinia YopH (residues 163-468) binds phosphonodifluoromethyl-Phe containing hexapeptide at two sites

PDB ID 1xxv

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