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| - | [[Image:1y0q.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of an active group I ribozyme-product complex== |
| - | |PDB= 1y0q |SIZE=350|CAPTION= <scene name='initialview01'>1y0q</scene>, resolution 3.60Å
| + | <StructureSection load='1y0q' size='340' side='right'caption='[[1y0q]], [[Resolution|resolution]] 3.60Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SPK:SPERMINE+(FULLY+PROTONATED+FORM)'>SPK</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[1y0q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_virus_Twort Staphylococcus virus Twort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y0Q FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | |GENE= orf142-I2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55510 Staphylococcus phage Twort])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SPK:SPERMINE+(FULLY+PROTONATED+FORM)'>SPK</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y0q OCA], [https://pdbe.org/1y0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y0q RCSB], [https://www.ebi.ac.uk/pdbsum/1y0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y0q ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y0q OCA], [http://www.ebi.ac.uk/pdbsum/1y0q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y0q RCSB]</span>
| + | |
| - | }}
| + | |
| | | | |
| - | '''Crystal structure of an active group I ribozyme-product complex'''
| + | ==See Also== |
| - | | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview==
| + | </StructureSection> |
| - | Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Staphylococcus virus Twort]] |
| - | ==About this Structure== | + | [[Category: Chase E]] |
| - | 1Y0Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA].
| + | [[Category: Golden BL]] |
| - | | + | [[Category: Kim H]] |
| - | ==Reference==
| + | |
| - | Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15580277 15580277]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Staphylococcus phage twort]] | + | |
| - | [[Category: Chase, E.]] | + | |
| - | [[Category: Golden, B L.]] | + | |
| - | [[Category: Kim, H.]] | + | |
| - | [[Category: guanosine-binding]]
| + | |
| - | [[Category: ribozyme]]
| + | |
| - | [[Category: rna-folding]]
| + | |
| - | [[Category: rna-splicing]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:57:16 2008''
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