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6b46
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1== | |
| + | <SX load='6b46' size='340' side='right' viewer='molstar' caption='[[6b46]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6b46]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa], [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14] and [https://en.wikipedia.org/wiki/Pseudomonas_phage_JBD30 Pseudomonas phage JBD30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B46 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b46 OCA], [https://pdbe.org/6b46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b46 RCSB], [https://www.ebi.ac.uk/pdbsum/6b46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b46 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CSY3_PSEAB CSY3_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[CRISPR subtype I-F|CRISPR subtype I-F]] | |
| - | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
| + | [[Category: Pseudomonas aeruginosa UCBPP-PA14]] | ||
| + | [[Category: Pseudomonas phage JBD30]] | ||
| + | [[Category: Bartesaghi A]] | ||
| + | [[Category: Earl L]] | ||
| + | [[Category: Falconieri V]] | ||
| + | [[Category: Fox T]] | ||
| + | [[Category: Guo TW]] | ||
| + | [[Category: Merk A]] | ||
| + | [[Category: Patel DJ]] | ||
| + | [[Category: Rao P]] | ||
| + | [[Category: Subramaniam S]] | ||
| + | [[Category: Yang H]] | ||
Current revision
Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1
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