6ek1

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'''Unreleased structure'''
 
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The entry 6ek1 is ON HOLD until Paper Publication
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==Crystal structure of Type IIP restriction endonuclease PfoI==
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<StructureSection load='6ek1' size='340' side='right'caption='[[6ek1]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6ek1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EK1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ek1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ek1 OCA], [https://pdbe.org/6ek1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ek1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ek1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ek1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A452CST7_PSEFL A0A452CST7_PSEFL]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Restriction endonucleases (REs) of the CCGG-family recognize a set of 4-8 bp target sequences that share a common CCGG or CCNGG core and possess PD...D/ExK nuclease fold. REs that interact with 5 bp sequence 5'-CCNGG flip the central N nucleotides and 'compress' the bound DNA to stack the inner base pairs to mimic the CCGG sequence. PfoI belongs to the CCGG-family and cleaves the 7 bp sequence 5'-T|CCNGGA ("|" designates cleavage position). We present here crystal structures of PfoI in free and DNA-bound forms that show unique active site arrangement and mechanism of sequence recognition. Structures and mutagenesis indicate that PfoI features a permuted E...ExD...K active site that differs from the consensus motif characteristic to other family members. Although PfoI also flips the central N nucleotides of the target sequence it does not 'compress' the bound DNA. Instead, PfoI induces a drastic change in DNA backbone conformation that shortens the distance between scissile phosphates to match that in the unperturbed CCGG sequence. Our data demonstrate the diversity and versatility of structural mechanisms employed by restriction enzymes for recognition of related DNA sequences.
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Authors: Tamulaitiene, G., Manakova, E., Jovaisaite, V., Grazulis, S., Siksnys, V.
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Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.,Tamulaitiene G, Manakova E, Jovaisaite V, Tamulaitis G, Grazulis S, Bochtler M, Siksnys V Nucleic Acids Res. 2019 Jan 25;47(2):997-1010. doi: 10.1093/nar/gky1137. PMID:30445642<ref>PMID:30445642</ref>
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Description: Crystal structure of Type IIP restriction endonuclease PfoI
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Manakova, E]]
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<div class="pdbe-citations 6ek1" style="background-color:#fffaf0;"></div>
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[[Category: Siksnys, V]]
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[[Category: Grazulis, S]]
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==See Also==
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[[Category: Tamulaitiene, G]]
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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[[Category: Jovaisaite, V]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas fluorescens]]
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[[Category: Grazulis S]]
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[[Category: Jovaisaite V]]
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[[Category: Manakova E]]
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[[Category: Siksnys V]]
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[[Category: Tamulaitiene G]]

Current revision

Crystal structure of Type IIP restriction endonuclease PfoI

PDB ID 6ek1

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