1y4o

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[[Image:1y4o.gif|left|200px]]
 
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{{Structure
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==Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain==
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|PDB= 1y4o |SIZE=350|CAPTION= <scene name='initialview01'>1y4o</scene>
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<StructureSection load='1y4o' size='340' side='right'caption='[[1y4o]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1y4o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4O FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= Dncl2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4o OCA], [https://pdbe.org/1y4o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4o RCSB], [https://www.ebi.ac.uk/pdbsum/1y4o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4o ProSAT], [https://www.topsan.org/Proteins/CESG/1y4o TOPSAN]</span></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam03259 Robl_LC7]</span>
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4o OCA], [http://www.ebi.ac.uk/pdbsum/1y4o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y4o RCSB]</span>
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[https://www.uniprot.org/uniprot/DLRB1_MOUSE DLRB1_MOUSE] Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y4/1y4o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y4o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Roadblock/LC7 is a member of a class of dynein light chains involved in regulating the function of the dynein complex. We have determined the three-dimensional structure of isoform 1 of the mouse Roadblock/LC7 cytoplasmic dynein light chain (robl1_mouse) by NMR spectroscopy. In contrast to a previously reported NMR structure of the human homolog with 96% sequence identity (PDB 1TGQ), which showed the protein as a monomer, our results indicate clearly that robl1 exists as a symmetric homodimer. The two beta3-strands pair with each other and form a continuous ten-stranded beta-sheet. The 25-residue alpha2-helix from one subunit packs antiparallel to that of the other subunit on the face of the beta-sheet. Zipper-like hydrophobic contacts between the two helices serve to stabilize the dimer. Through an NMR titration experiment, we localized the site on robl1_mouse that interacts with the 40 residue peptide spanning residues 243 through 282 of IC74-1_rat. These results provide physical evidence for a symmetrical interaction between dimeric robl1 and the two molecules of IC74-1 in the dynein complex.
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'''Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain'''
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Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex.,Song J, Tyler RC, Lee MS, Tyler EM, Markley JL J Mol Biol. 2005 Dec 16;354(5):1043-51. Epub 2005 Nov 2. PMID:16289575<ref>PMID:16289575</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1y4o" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Roadblock/LC7 is a member of a class of dynein light chains involved in regulating the function of the dynein complex. We have determined the three-dimensional structure of isoform 1 of the mouse Roadblock/LC7 cytoplasmic dynein light chain (robl1_mouse) by NMR spectroscopy. In contrast to a previously reported NMR structure of the human homolog with 96% sequence identity (PDB 1TGQ), which showed the protein as a monomer, our results indicate clearly that robl1 exists as a symmetric homodimer. The two beta3-strands pair with each other and form a continuous ten-stranded beta-sheet. The 25-residue alpha2-helix from one subunit packs antiparallel to that of the other subunit on the face of the beta-sheet. Zipper-like hydrophobic contacts between the two helices serve to stabilize the dimer. Through an NMR titration experiment, we localized the site on robl1_mouse that interacts with the 40 residue peptide spanning residues 243 through 282 of IC74-1_rat. These results provide physical evidence for a symmetrical interaction between dimeric robl1 and the two molecules of IC74-1 in the dynein complex.
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*[[Dynein 3D structures|Dynein 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1Y4O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4O OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex., Song J, Tyler RC, Lee MS, Tyler EM, Markley JL, J Mol Biol. 2005 Dec 16;354(5):1043-51. Epub 2005 Nov 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16289575 16289575]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Lee MS]]
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[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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[[Category: Markley JL]]
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[[Category: Lee, M S.]]
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[[Category: Song J]]
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[[Category: Markley, J L.]]
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[[Category: Tyler EM]]
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[[Category: Song, J.]]
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[[Category: Tyler RC]]
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[[Category: Tyler, E M.]]
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[[Category: Tyler, R C.]]
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[[Category: center for eukaryotic structural genomic]]
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[[Category: cesg]]
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[[Category: dynein light chain]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:44 2008''
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Current revision

Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain

PDB ID 1y4o

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