5c8j

Publication Abstract from PubMed

Cells possess specialized machinery to direct the insertion of membrane proteins into the lipid bilayer. In bacteria, the essential protein YidC inserts certain proteins into the plasma membrane, and eukaryotic orthologs are present in the mitochondrial inner membrane and the chloroplast thylakoid membrane. The existence of homologous insertases in archaea has been proposed based on phylogenetic analysis. However, limited sequence identity, distinct architecture, and the absence of experimental data have made this assignment ambiguous. Here we describe the 3.5-A crystal structure of an archaeal DUF106 protein from Methanocaldococcus jannaschii (Mj0480), revealing a lipid-exposed hydrophilic surface presented by a conserved YidC-like fold. Functional analysis reveals selective binding of Mj0480 to ribosomes displaying a stalled YidC substrate, and a direct interaction between the buried hydrophilic surface of Mj0480 and the nascent chain. These data provide direct experimental evidence that the archaeal DUF106 proteins are YidC/Oxa1/Alb3-like insertases of the archaeal plasma membrane.

A YidC-like Protein in the Archaeal Plasma Membrane.,Borowska MT, Dominik PK, Anghel SA, Kossiakoff AA, Keenan RJ Structure. 2015 Aug 5. pii: S0969-2126(15)00278-6. doi:, 10.1016/j.str.2015.06.025. PMID:26256539^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.