5dn7

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==Crescerin uses a TOG domain array to regulate microtubules in the primary cilium==
==Crescerin uses a TOG domain array to regulate microtubules in the primary cilium==
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<StructureSection load='5dn7' size='340' side='right' caption='[[5dn7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='5dn7' size='340' side='right'caption='[[5dn7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5dn7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DN7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DN7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5dn7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DN7 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dn7 OCA], [http://pdbe.org/5dn7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dn7 RCSB], [http://www.ebi.ac.uk/pdbsum/5dn7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dn7 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dn7 OCA], [https://pdbe.org/5dn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dn7 RCSB], [https://www.ebi.ac.uk/pdbsum/5dn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dn7 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TGRM1_MOUSE TGRM1_MOUSE] Involved in ciliogenesis. It is required for appropriate acetylation and polyglutamylation of ciliary microtubules, and regulation of cilium length (By similarity). Interacts with microtubules and promotes microtubule polymerization via its HEAT repeat domains, especially those in TOG region 2 and 4 (PubMed:26378256, PubMed:32747439).[UniProtKB:Q17423]<ref>PMID:26378256</ref> <ref>PMID:32747439</ref>
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Eukaryotic cilia are cell-surface projections, critical for sensing the extracellular environment. Defects in cilia structure and function result in a broad range of developmental and sensory disorders. However, mechanisms that regulate the microtubule (MT)-based scaffold forming the cilia core are poorly understood. TOG domain array-containing proteins ch-TOG and CLASP are key regulators of cytoplasmic MTs. Whether TOG array proteins also regulate ciliary MTs is unknown. Here, we have identified the conserved Crescerin protein family as a cilia-specific TOG array-containing MT regulator. We present the crystal structure of mammalian Crescerin1 TOG2, revealing a canonical TOG fold with conserved tubulin-binding determinants. Crescerin1's TOG domains possess inherent MT-binding activity and promote MT polymerization in vitro. Using Cas9-triggered homologous recombination in Caenorhabditis elegans, we demonstrate that the worm Crescerin family member CHE-12 requires TOG domain-dependent tubulin-binding activity for sensory cilia development. Thus, Crescerin expands the TOG domain array-based MT regulatory paradigm beyond ch-TOG and CLASP, representing a distinct regulator of cilia structure.
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Crescerin uses a TOG domain array to regulate microtubules in the primary cilium.,Das A, Dickinson DJ, Wood CC, Goldstein B, Slep KC Mol Biol Cell. 2015 Sep 16. pii: mbc.E15-08-0603. PMID:26378256<ref>PMID:26378256</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5dn7" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Das, A]]
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[[Category: Large Structures]]
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[[Category: Dickinson, D J]]
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[[Category: Mus musculus]]
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[[Category: Goldstein, B]]
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[[Category: Das A]]
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[[Category: Slep, K C]]
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[[Category: Dickinson DJ]]
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[[Category: Wood, C C]]
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[[Category: Goldstein B]]
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[[Category: Structural protein]]
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[[Category: Slep KC]]
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[[Category: Tog domain]]
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[[Category: Wood CC]]

Current revision

Crescerin uses a TOG domain array to regulate microtubules in the primary cilium

PDB ID 5dn7

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