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| | ==Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor== | | ==Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor== |
| - | <StructureSection load='5iw7' size='340' side='right' caption='[[5iw7]], [[Resolution|resolution]] 3.60Å' scene=''> | + | <StructureSection load='5iw7' size='340' side='right'caption='[[5iw7]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5iw7]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IW7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IW7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5iw7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IW7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iw7 OCA], [http://pdbe.org/5iw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iw7 RCSB], [http://www.ebi.ac.uk/pdbsum/5iw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iw7 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iw7 OCA], [https://pdbe.org/5iw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iw7 RCSB], [https://www.ebi.ac.uk/pdbsum/5iw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iw7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TSR1_YEAST TSR1_YEAST]] Required for 40S ribosomal subunit synthesis. Required for normal export of the pre-40S particles from the nucleus to the cytoplasm. Its subcellular location and association with pre-40S subunitshifts from mixed cytoplasm/nucleus to all nuclear in RPS19 disruptions, suggesting it acts after the ribosomal protein.<ref>PMID:11565749</ref> <ref>PMID:12628929</ref> <ref>PMID:15167894</ref> <ref>PMID:16159874</ref> | + | [https://www.uniprot.org/uniprot/TSR1_YEAST TSR1_YEAST] Required for 40S ribosomal subunit synthesis. Required for normal export of the pre-40S particles from the nucleus to the cytoplasm. Its subcellular location and association with pre-40S subunitshifts from mixed cytoplasm/nucleus to all nuclear in RPS19 disruptions, suggesting it acts after the ribosomal protein.<ref>PMID:11565749</ref> <ref>PMID:12628929</ref> <ref>PMID:15167894</ref> <ref>PMID:16159874</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5iw7" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5iw7" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cook, A G]] | + | [[Category: Large Structures]] |
| - | [[Category: Jayachandran, U]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: McCaughan, U M]] | + | [[Category: Cook AG]] |
| - | [[Category: Translation]] | + | [[Category: Jayachandran U]] |
| - | [[Category: Translational gtpase ribosome synthesis]] | + | [[Category: McCaughan UM]] |
| Structural highlights
Function
TSR1_YEAST Required for 40S ribosomal subunit synthesis. Required for normal export of the pre-40S particles from the nucleus to the cytoplasm. Its subcellular location and association with pre-40S subunitshifts from mixed cytoplasm/nucleus to all nuclear in RPS19 disruptions, suggesting it acts after the ribosomal protein.[1] [2] [3] [4]
Publication Abstract from PubMed
Budding yeast Tsr1 is a ribosome biogenesis factor with sequence similarity to GTPases, which is essential for cytoplasmic steps in 40S subunit maturation. Here we present the crystal structure of Tsr1 at 3.6 A. Tsr1 has a similar domain architecture to translational GTPases such as EF-Tu and the selenocysteine incorporation factor SelB. However, active site residues required for GTP binding and hydrolysis are absent, explaining the lack of enzymatic activity in previous analyses. Modelling of Tsr1 into cryo-electron microscopy maps of pre-40S particles shows that a highly acidic surface of Tsr1 is presented on the outside of pre-40S particles, potentially preventing premature binding to 60S subunits. Late pre-40S maturation also requires the GTPase eIF5B and the ATPase Rio1. The location of Tsr1 is predicted to block binding by both factors, strongly indicating that removal of Tsr1 is an essential step during cytoplasmic maturation of 40S ribosomal subunits.
Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases.,McCaughan UM, Jayachandran U, Shchepachev V, Chen ZA, Rappsilber J, Tollervey D, Cook AG Nat Commun. 2016 Jun 2;7:11789. doi: 10.1038/ncomms11789. PMID:27250689[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Gelperin D, Horton L, Beckman J, Hensold J, Lemmon SK. Bms1p, a novel GTP-binding protein, and the related Tsr1p are required for distinct steps of 40S ribosome biogenesis in yeast. RNA. 2001 Sep;7(9):1268-83. PMID:11565749
- ↑ Schafer T, Strauss D, Petfalski E, Tollervey D, Hurt E. The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes. EMBO J. 2003 Mar 17;22(6):1370-80. PMID:12628929 doi:http://dx.doi.org/10.1093/emboj/cdg121
- ↑ Leger-Silvestre I, Milkereit P, Ferreira-Cerca S, Saveanu C, Rousselle JC, Choesmel V, Guinefoleau C, Gas N, Gleizes PE. The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast. EMBO J. 2004 Jun 16;23(12):2336-47. Epub 2004 May 27. PMID:15167894 doi:http://dx.doi.org/10.1038/sj.emboj.7600252
- ↑ Leger-Silvestre I, Caffrey JM, Dawaliby R, Alvarez-Arias DA, Gas N, Bertolone SJ, Gleizes PE, Ellis SR. Specific Role for Yeast Homologs of the Diamond Blackfan Anemia-associated Rps19 Protein in Ribosome Synthesis. J Biol Chem. 2005 Nov 18;280(46):38177-85. Epub 2005 Sep 12. PMID:16159874 doi:http://dx.doi.org/10.1074/jbc.M506916200
- ↑ McCaughan UM, Jayachandran U, Shchepachev V, Chen ZA, Rappsilber J, Tollervey D, Cook AG. Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases. Nat Commun. 2016 Jun 2;7:11789. doi: 10.1038/ncomms11789. PMID:27250689 doi:http://dx.doi.org/10.1038/ncomms11789
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