1yfn

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[[Image:1yfn.gif|left|200px]]
 
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{{Structure
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==Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex==
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|PDB= 1yfn |SIZE=350|CAPTION= <scene name='initialview01'>1yfn</scene>, resolution 1.80&Aring;
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<StructureSection load='1yfn' size='340' side='right'caption='[[1yfn]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1yfn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YFN FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yfn OCA], [https://pdbe.org/1yfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yfn RCSB], [https://www.ebi.ac.uk/pdbsum/1yfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yfn ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1ou8|1OU8]], [[1ou9|1OU9]], [[1oul|1OUL]], [[1ox8|1OX8]], [[1ox9|1OX9]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yfn OCA], [http://www.ebi.ac.uk/pdbsum/1yfn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yfn RCSB]</span>
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[https://www.uniprot.org/uniprot/SSPB_ECOLI SSPB_ECOLI] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.<ref>PMID:11009422</ref> <ref>PMID:15371343</ref> <ref>PMID:12887894</ref> Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion.<ref>PMID:11009422</ref> <ref>PMID:15371343</ref> <ref>PMID:12887894</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yf/1yfn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yfn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Energy-dependent proteases often rely on adaptor proteins to modulate substrate recognition. The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the AAA+ ClpXP protease for degradation. The structure of SspB bound to an ssrA peptide is known. Here, we report the crystal structure of a complex between SspB and its recognition peptide in RseA. Notably, the RseA sequence is positioned in the peptide-binding groove of SspB in a direction opposite to the ssrA peptide, the two peptides share only one common interaction with the adaptor, and the RseA interaction site is substantially larger than the overlapping ssrA site. This marked diversity in SspB recognition of different target proteins indicates that it is capable of highly flexible and dynamic substrate delivery.
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'''Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex'''
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Versatile modes of peptide recognition by the AAA+ adaptor protein SspB.,Levchenko I, Grant RA, Flynn JM, Sauer RT, Baker TA Nat Struct Mol Biol. 2005 Jun;12(6):520-5. Epub 2005 May 8. PMID:15880122<ref>PMID:15880122</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1yfn" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Energy-dependent proteases often rely on adaptor proteins to modulate substrate recognition. The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the AAA+ ClpXP protease for degradation. The structure of SspB bound to an ssrA peptide is known. Here, we report the crystal structure of a complex between SspB and its recognition peptide in RseA. Notably, the RseA sequence is positioned in the peptide-binding groove of SspB in a direction opposite to the ssrA peptide, the two peptides share only one common interaction with the adaptor, and the RseA interaction site is substantially larger than the overlapping ssrA site. This marked diversity in SspB recognition of different target proteins indicates that it is capable of highly flexible and dynamic substrate delivery.
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*[[Stringent starvation protein 3D structures|Stringent starvation protein 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1YFN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YFN OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Versatile modes of peptide recognition by the AAA+ adaptor protein SspB., Levchenko I, Grant RA, Flynn JM, Sauer RT, Baker TA, Nat Struct Mol Biol. 2005 Jun;12(6):520-5. Epub 2005 May 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15880122 15880122]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Baker, T A.]]
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[[Category: Baker TA]]
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[[Category: Flynn, J M.]]
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[[Category: Flynn JM]]
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[[Category: Grant, R A.]]
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[[Category: Grant RA]]
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[[Category: Levchenko, I.]]
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[[Category: Levchenko I]]
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[[Category: Sauer, R T.]]
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[[Category: Sauer RT]]
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[[Category: protein-peptide complex]]
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[[Category: rsea]]
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[[Category: sspb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:05:51 2008''
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Current revision

Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex

PDB ID 1yfn

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