1ygm

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[[Image:1ygm.gif|left|200px]]
 
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{{Structure
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==NMR structure of Mistic==
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|PDB= 1ygm |SIZE=350|CAPTION= <scene name='initialview01'>1ygm</scene>
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<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [https://pdbe.org/1ygm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [https://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB]</span>
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[https://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref>
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}}
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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'''NMR structure of Mistic'''
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==Overview==
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Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
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==About this Structure==
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NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.,Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457<ref>PMID:15731457</ref>
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1YGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15731457 15731457]
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</div>
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<div class="pdbe-citations 1ygm" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Castronovo, S.]]
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[[Category: Castronovo S]]
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[[Category: Choe, S.]]
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[[Category: Choe S]]
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[[Category: Greenwald, J.]]
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[[Category: Greenwald J]]
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[[Category: Riek, R.]]
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[[Category: Riek R]]
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[[Category: Roosild, T P.]]
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[[Category: Roosild TP]]
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[[Category: Vega, M.]]
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[[Category: Vega M]]
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[[Category: alpha-helical bundle]]
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[[Category: integral membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:06:38 2008''
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Current revision

NMR structure of Mistic

PDB ID 1ygm

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