1yk3

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[[Image:1yk3.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Rv1347c from Mycobacterium tuberculosis==
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|PDB= 1yk3 |SIZE=350|CAPTION= <scene name='initialview01'>1yk3</scene>, resolution 2.20&Aring;
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<StructureSection load='1yk3' size='340' side='right'caption='[[1yk3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>
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<table><tr><td colspan='2'>[[1yk3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YK3 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= Rv1347c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG1670 RimL]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yk3 OCA], [https://pdbe.org/1yk3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yk3 RCSB], [https://www.ebi.ac.uk/pdbsum/1yk3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yk3 ProSAT], [https://www.topsan.org/Proteins/TBSGC/1yk3 TOPSAN]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yk3 OCA], [http://www.ebi.ac.uk/pdbsum/1yk3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yk3 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MBTK_MYCTU MBTK_MYCTU] Acyltransferase required for the direct transfer of medium- to long-chain fatty acyl moieties from a carrier protein (MbtL) on to the epsilon-amino group of lysine residue in the mycobactin core.<ref>PMID:16461464</ref>
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== Evolutionary Conservation ==
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'''Crystal structure of Rv1347c from Mycobacterium tuberculosis'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yk/1yk3_consurf.spt"</scriptWhenChecked>
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Mycobacterium tuberculosis, the cause of tuberculosis, is a devastating human pathogen. The emergence of multidrug resistance in recent years has prompted a search for new drug targets and for a better understanding of mechanisms of resistance. Here we focus on the gene product of an open reading frame from M. tuberculosis, Rv1347c, which is annotated as a putative aminoglycoside N-acetyltransferase. The Rv1347c protein does not show this activity, however, and we show from its crystal structure, coupled with functional and bioinformatic data, that its most likely role is in the biosynthesis of mycobactin, the M. tuberculosis siderophore. The crystal structure of Rv1347c was determined by multiwavelength anomalous diffraction phasing from selenomethionine-substituted protein and refined at 2.2 angstrom resolution (r = 0.227, R(free) = 0.257). The protein is monomeric, with a fold that places it in the GCN5-related N-acetyltransferase (GNAT) family of acyltransferases. Features of the structure are an acyl-CoA binding site that is shared with other GNAT family members and an adjacent hydrophobic channel leading to the surface that could accommodate long-chain acyl groups. Modeling the postulated substrate, the N(epsilon)-hydroxylysine side chain of mycobactin, into the acceptor substrate binding groove identifies two residues at the active site, His130 and Asp168, that have putative roles in substrate binding and catalysis.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1YK3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK3 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yk3 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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The crystal structure of Rv1347c, a putative antibiotic resistance protein from Mycobacterium tuberculosis, reveals a GCN5-related fold and suggests an alternative function in siderophore biosynthesis., Card GL, Peterson NA, Smith CA, Rupp B, Schick BM, Baker EN, J Biol Chem. 2005 Apr 8;280(14):13978-86. Epub 2005 Jan 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15695811 15695811]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Baker EN]]
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[[Category: Baker, E N.]]
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[[Category: Card GL]]
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[[Category: Card, G L.]]
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[[Category: Peterson NA]]
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[[Category: Peterson, N A.]]
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[[Category: Rupp B]]
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[[Category: Rupp, B.]]
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[[Category: Schick BM]]
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[[Category: Schick, B M.]]
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[[Category: Smith CA]]
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[[Category: Smith, C A.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: acyltransferase]]
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[[Category: gcn5-related fold]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomic]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:10:20 2008''
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Current revision

Crystal structure of Rv1347c from Mycobacterium tuberculosis

PDB ID 1yk3

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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