1mqk

<StructureSection load='1mqk' size='340' side='right' caption='[[1mqk]], [[Resolution|resolution]] 1.28&Aring;' scene=''>

<table><tr><td colspan='2'>[[1mqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MQK FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ar1|1ar1]], [[1qle|1qle]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mqk OCA], [http://pdbe.org/1mqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mqk RCSB], [http://www.ebi.ac.uk/pdbsum/1mqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mqk ProSAT]</span></td></tr>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mq/1mqk_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

The antibody Fv fragment 7E2 has previously been employed in the induced crystallization of the integral membrane protein cytochrome c oxidase from Paracoccus denitrificans. The 1.3 A X-ray structure of the uncomplexed antibody fragment reveals conserved water networks on the surfaces of the framework regions. A novel consensus motif for water coordination, XX(S/T), is found along the edges of the beta-sandwich, where a water molecule forms hydrogen bonds to the carbonyl O atom of a residue at position N and the OG hydroxyl groups of conserved serines or threonines at position N + 2. Multiple conformations were found in the hydrophobic core for residues IleL21, LeuL33 and the disulfide bridges. An internal water molecule that is compatible with only one of the three packing states of the V(L) core suggests local 'breathing' of the variable domain. TrpH47, a conserved key residue of the V(H)/V(L) interface, is crucially involved in the formation of the antigen-binding site by adopting a novel conformation that specifically stabilizes the non-canonical CDR-L3 loop. Finally, a comparison with 7E2-cytochrome c oxidase complexes demonstrates that binding of this membrane-bound antigen proceeds without major conformational changes of the 7E2 antibody fragment.

1.3 A X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization.,Essen LO, Harrenga A, Ostermeier C, Michel H Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):677-87. Epub 2003, Mar 25. PMID:12657787<ref>PMID:12657787</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1mqk" style="background-color:#fffaf0;"></div>

*[[3D structures of monoclonal antibody|3D structures of monoclonal antibody]]

[[Category: Lk3 transgenic mice]]

[[Category: Essen, L O]]

[[Category: Harrenga, A]]

[[Category: Michel, H]]

[[Category: Ostermeier, C]]

[[Category: Membrane protein]]