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1wvn

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Crystal Structure of domain 3 of human alpha polyC binding protein

Structural highlights

1wvn is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PCBP1_HUMAN Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Poly(C)-binding proteins (CPs) are important regulators of mRNA stability and translational regulation. They recognize C-rich RNA through their triple KH (hn RNP K homology) domain structures and are thought to carry out their function though direct protection of mRNA sites as well as through interactions with other RNA-binding proteins. We report the crystallographically derived structure of the third domain of alphaCP1 to 2.1 A resolution. alphaCP1-KH3 assumes a classical type I KH domain fold with a triple-stranded beta-sheet held against a three-helix cluster in a betaalphaalphabetabetaalpha configuration. Its binding affinity to an RNA sequence from the 3'-untranslated region (3'-UTR) of androgen receptor mRNA was determined using surface plasmon resonance, giving a K(d) of 4.37 microM, which is indicative of intermediate binding. A model of alphaCP1-KH3 with poly(C)-RNA was generated by homology to a recently reported RNA-bound KH domain structure and suggests the molecular basis for oligonucleotide binding and poly(C)-RNA specificity.

Structure and RNA binding of the third KH domain of poly(C)-binding protein 1.,Sidiqi M, Wilce JA, Vivian JP, Porter CJ, Barker A, Leedman PJ, Wilce MC Nucleic Acids Res. 2005 Feb 24;33(4):1213-21. Print 2005. PMID:15731341^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sidiqi M, Wilce JA, Vivian JP, Porter CJ, Barker A, Leedman PJ, Wilce MC. Structure and RNA binding of the third KH domain of poly(C)-binding protein 1. Nucleic Acids Res. 2005 Feb 24;33(4):1213-21. Print 2005. PMID:15731341 doi:33/4/1213

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wvn"

Categories: Homo sapiens | Large Structures | Sidiqu M | Wilce JA | Wilce MCJ

@@ Line 1: / Line 1: @@
-==Crsytal Structure of domain 3 of human alpha polyC binding protein==
+==Crystal Structure of domain 3 of human alpha polyC binding protein==
-<StructureSection load='1wvn' size='340' side='right' caption='[[1wvn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='1wvn' size='340' side='right'caption='[[1wvn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1wvn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WVN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1wvn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WVN FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvn OCA], [http://pdbe.org/1wvn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wvn RCSB], [http://www.ebi.ac.uk/pdbsum/1wvn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvn ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvn OCA], [https://pdbe.org/1wvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wvn RCSB], [https://www.ebi.ac.uk/pdbsum/1wvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PCBP1_HUMAN PCBP1_HUMAN]] Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
+[https://www.uniprot.org/uniprot/PCBP1_HUMAN PCBP1_HUMAN] Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wv/1wvn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wv/1wvn_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Sidiqu, M]]
+[[Category: Large Structures]]
-[[Category: Wilce, J A]]
+[[Category: Sidiqu M]]
-[[Category: Wilce, M C.J]]
+[[Category: Wilce JA]]
-[[Category: Kh domain]]
+[[Category: Wilce MCJ]]
-[[Category: Rna binding domain]]
-[[Category: Rna binding protein]]

1wvn

From Proteopedia

Current revision

Crystal Structure of domain 3 of human alpha polyC binding protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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