1r71

<StructureSection load='1r71' size='340' side='right' caption='[[1r71]], [[Resolution|resolution]] 2.20&Aring;' scene=''>

<table><tr><td colspan='2'>[[1r71]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R71 FirstGlance]. <br>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KORB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r71 OCA], [http://pdbe.org/1r71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r71 RCSB], [http://www.ebi.ac.uk/pdbsum/1r71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r71 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/KORB2_ECOLX KORB2_ECOLX]] In conjunction with KorA, inhibits the transcription of the kilA, trfA and korAB operons. Is also involved in the negative control of the kilB operon.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r7/1r71_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

The KorB protein of the broad-host-range plasmid RP4 acts as a multifunctional regulator of plasmid housekeeping genes, including those responsible for replication, maintenance and conjugation. Additionally, KorB functions as the ParB analog of the plasmid's partitioning system. The protein structure consists of eight helices, two of which belong to a predicted helix-turn-helix motif. Each half-site of the palindromic operator DNA binds one copy of the protein in the major groove. As confirmed by mutagenesis, recognition specificity is based mainly on two side chain interactions outside the helix-turn-helix motif with two bases next to the central base pair of the 13-base pair operator sequence. The surface of the KorB DNA-binding domain mirrors the overall acidity of KorB, whereas DNA binding occurs via a basic interaction surface. We present a model of KorB, including the structure of its dimerization domain, and discuss its interactions with the highly basic ParA homolog IncC.

Sequence-specific DNA binding determined by contacts outside the helix-turn-helix motif of the ParB homolog KorB.,Khare D, Ziegelin G, Lanka E, Heinemann U Nat Struct Mol Biol. 2004 Jul;11(7):656-63. Epub 2004 May 30. PMID:15170177<ref>PMID:15170177</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1r71" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Bacillus coli migula 1895]]

[[Category: Heinemann, U]]

[[Category: Khare, D]]

[[Category: Lanka, E]]

[[Category: Ziegelin, G]]

[[Category: Transcription-dna complex]]