2d2a

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Escherichia coli SufA Involved in Biosynthesis of Iron-sulfur Clusters

Structural highlights

2d2a is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SUFA_ECOLI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

IscA and SufA are paralogous proteins that play crucial roles in the biosynthesis of Fe-S clusters, perhaps through a mechanism involving transient Fe-S cluster formation. We have determined the crystal structure of E. coli SufA at 2.7A resolution. SufA exists as a homodimer, in contrast to the tetrameric organization of IscA. Furthermore, a C-terminal segment containing two essential cysteine residues (Cys-Gly-Cys), which is disordered in the IscA structure, is clearly visible in one molecule (the alpha1 subunit) of the SufA homodimer. Although this segment is disordered in the other molecule (the alpha2 subunit), computer modeling of this segment based on the well-defined conformation of alpha1 subunit suggests that the four cysteine residues (Cys114 and Cys116 in each subunit) in the Cys-Gly-Cys motif are positioned in close proximity at the dimer interface. The arrangement of these cysteines together with the nearby Glu118 in SufA dimer may allow coordination of an Fe-S cluster and/or an Fe atom.

Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer.,Wada K, Hasegawa Y, Gong Z, Minami Y, Fukuyama K, Takahashi Y FEBS Lett. 2005 Dec 5;579(29):6543-8. Epub 2005 Nov 15. PMID:16298366^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wada K, Hasegawa Y, Gong Z, Minami Y, Fukuyama K, Takahashi Y. Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer. FEBS Lett. 2005 Dec 5;579(29):6543-8. Epub 2005 Nov 15. PMID:16298366 doi:10.1016/j.febslet.2005.10.046

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2d2a"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Escherichia coli SufA Involved in Biosynthesis of Iron-sulfur Clusters==
-<StructureSection load='2d2a' size='340' side='right' caption='[[2d2a]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='2d2a' size='340' side='right'caption='[[2d2a]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2d2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D2A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2d2a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D2A FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d2a OCA], [http://pdbe.org/2d2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d2a RCSB], [http://www.ebi.ac.uk/pdbsum/2d2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d2a ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d2a OCA], [https://pdbe.org/2d2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d2a RCSB], [https://www.ebi.ac.uk/pdbsum/2d2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d2a ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SUFA_ECOLI SUFA_ECOLI]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/2d2a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/2d2a_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Fukuyama, K]]
+[[Category: Large Structures]]
-[[Category: Gong, Z]]
+[[Category: Fukuyama K]]
-[[Category: Hasegawa, Y]]
+[[Category: Gong Z]]
-[[Category: Minami, Y]]
+[[Category: Hasegawa Y]]
-[[Category: Takahashi, Y]]
+[[Category: Minami Y]]
-[[Category: Wada, K]]
+[[Category: Takahashi Y]]
-[[Category: Iron]]
+[[Category: Wada K]]
-[[Category: Iron-sulfur cluster]]
-[[Category: Isca]]
-[[Category: Metal transport]]
-[[Category: Suf]]
-[[Category: Sufa]]
-[[Category: Yadr]]

2d2a

From Proteopedia

Current revision

Crystal Structure of Escherichia coli SufA Involved in Biosynthesis of Iron-sulfur Clusters

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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