1ynt

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[[Image:1ynt.gif|left|200px]]
 
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{{Structure
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==Structure of the monomeric form of T. gondii SAG1 surface antigen bound to a human Fab==
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|PDB= 1ynt |SIZE=350|CAPTION= <scene name='initialview01'>1ynt</scene>, resolution 3.100&Aring;
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<StructureSection load='1ynt' size='340' side='right'caption='[[1ynt]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>
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<table><tr><td colspan='2'>[[1ynt]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna_ATCC_29328 Finegoldia magna ATCC 29328], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNT FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ynt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynt OCA], [https://pdbe.org/1ynt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ynt RCSB], [https://www.ebi.ac.uk/pdbsum/1ynt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ynt ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1kzq|1KZQ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ynt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynt OCA], [http://www.ebi.ac.uk/pdbsum/1ynt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ynt RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q51918_FINMA Q51918_FINMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1ynt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ynt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.
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'''Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody'''
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Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response.,Graille M, Stura EA, Bossus M, Muller BH, Letourneur O, Battail-Poirot N, Sibai G, Gauthier M, Rolland D, Le Du MH, Ducancel F J Mol Biol. 2005 Nov 25;354(2):447-58. Epub 2005 Sep 30. PMID:16242717<ref>PMID:16242717</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ynt" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1YNT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Finegoldia magna ATCC 29328]]
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Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response., Graille M, Stura EA, Bossus M, Muller BH, Letourneur O, Battail-Poirot N, Sibai G, Gauthier M, Rolland D, Le Du MH, Ducancel F, J Mol Biol. 2005 Nov 25;354(2):447-58. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16242717 16242717]
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[[Category: Large Structures]]
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[[Category: Finegoldia magna]]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
 
[[Category: Toxoplasma gondii]]
[[Category: Toxoplasma gondii]]
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[[Category: Battail-Poirot, N]]
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[[Category: Battail-Poirot N]]
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[[Category: Bossus, M.]]
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[[Category: Bossus M]]
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[[Category: Du, M H.Le.]]
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[[Category: Ducancel F]]
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[[Category: Ducancel, F.]]
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[[Category: Graille M]]
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[[Category: Graille, M.]]
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[[Category: Le Du MH]]
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[[Category: Letourneur, O.]]
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[[Category: Letourneur O]]
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[[Category: Muller, B H.]]
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[[Category: Muller BH]]
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[[Category: Rolland, D.]]
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[[Category: Rolland D]]
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[[Category: Sibai, G.]]
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[[Category: Sibai G]]
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[[Category: Stura, E A.]]
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[[Category: Stura EA]]
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[[Category: conformational epitope]]
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[[Category: crystal structure]]
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[[Category: recombinant sag1]]
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[[Category: toxoplasma gondii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:14:50 2008''
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Current revision

Structure of the monomeric form of T. gondii SAG1 surface antigen bound to a human Fab

PDB ID 1ynt

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