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5gxt

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==Crystal structure of PigG==
==Crystal structure of PigG==
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<StructureSection load='5gxt' size='340' side='right' caption='[[5gxt]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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<StructureSection load='5gxt' size='340' side='right'caption='[[5gxt]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5gxt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GXT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5gxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Serratia_sp._FS14 Serratia sp. FS14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GXT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.245&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gxv|5gxv]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gxt OCA], [http://pdbe.org/5gxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gxt RCSB], [http://www.ebi.ac.uk/pdbsum/5gxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gxt ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gxt OCA], [https://pdbe.org/5gxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gxt RCSB], [https://www.ebi.ac.uk/pdbsum/5gxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gxt ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
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[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Prodigiosin, a tripyrrole red pigment is synthesized by Serratia and some other microbes through a bifurcated biosynthesis pathway; MBC (4-methoxy-2,2'-bipyrrole-5-carbaldehyde) and MAP (2-methyl-3-n-amyl-pyrrole) are synthesized separately and then condensed by PigC to form prodigiosin. PigI, PigG and PigA have been shown to be involved in the first steps of MBC biosynthesis (proline incorporation). The crystal structure of PigG was resolved to elucidate its function and mechanism. PigG, an acyl carrier protein (ACP), features the ACP architecture:, a helical bundle fold containing three major helices and a minor distorted helix together with a conserved "S" motif. An in-frame deletion mutation of the pigG gene abolished the synthesis of prodigiosin in Serratia marcescens FS14. The production of prodigiosin was fully restored by complementation of intact pigG; however the S36A mutant was not able to restore function in the in-frame deletion pigG mutant, indicating that PigG and the conserved serine residue (S36) of PigG are essential for the synthesis of prodigiosin.
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Crystal structure of MBP-PigG fusion protein and the essential function of PigG in the prodigiosin biosynthetic pathway in Serratia marcescens FS14.,Zhang F, Wei Q, Tong H, Xu D, Wang W, Ran T Int J Biol Macromol. 2017 Jun;99:394-400. doi: 10.1016/j.ijbiomac.2017.02.088., Epub 2017 Mar 1. PMID:28258005<ref>PMID:28258005</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5gxt" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ran, T]]
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[[Category: Escherichia coli K-12]]
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[[Category: Wang, W]]
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[[Category: Large Structures]]
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[[Category: Xu, D]]
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[[Category: Serratia sp. FS14]]
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[[Category: Zhang, F]]
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[[Category: Ran T]]
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[[Category: Pigg]]
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[[Category: Wang W]]
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[[Category: Prodigiosin]]
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[[Category: Xu D]]
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[[Category: Protein transport]]
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[[Category: Zhang F]]

Current revision

Crystal structure of PigG

PDB ID 5gxt

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