5v8e
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==Structure of Bacillus cereus PatB1== | ==Structure of Bacillus cereus PatB1== | ||
| - | <StructureSection load='5v8e' size='340' side='right' caption='[[5v8e]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='5v8e' size='340' side='right'caption='[[5v8e]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5v8e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8E OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5v8e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_10987 Bacillus cereus ATCC 10987]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V8E FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8e OCA], [https://pdbe.org/5v8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v8e RCSB], [https://www.ebi.ac.uk/pdbsum/5v8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8e ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q73CU0_BACC1 Q73CU0_BACC1] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1. | ||
| + | |||
| + | PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.,Sychantha D, Little DJ, Chapman RN, Boons GJ, Robinson H, Howell PL, Clarke AJ Nat Chem Biol. 2017 Oct 30. pii: nchembio.2509. doi: 10.1038/nchembio.2509. PMID:29083419<ref>PMID:29083419</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5v8e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Bacillus cereus ATCC 10987]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Boons GJ]] |
| - | [[Category: | + | [[Category: Chapman RN]] |
| - | [[Category: | + | [[Category: Clarke AJ]] |
| - | [[Category: | + | [[Category: Howell PL]] |
| - | [[Category: | + | [[Category: Little DJ]] |
| - | [[Category: | + | [[Category: Robinson H]] |
| - | [[Category: | + | [[Category: Sychantha D]] |
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Current revision
Structure of Bacillus cereus PatB1
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