2h7w

<StructureSection load='2h7w' size='340' side='right' caption='[[2h7w]], [[Resolution|resolution]] 1.70&Aring;' scene=''>

<table><tr><td colspan='2'>[[2h7w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7W FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5693 TRYCR])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7w OCA], [http://pdbe.org/2h7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h7w RCSB], [http://www.ebi.ac.uk/pdbsum/2h7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7w ProSAT]</span></td></tr>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/2h7w_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

Trypanosoma cruzi chagasin belongs to a recently discovered family of cysteine protease inhibitors found in lower eukaryotes and prokaryotes but not in mammals. Chagasin binds tightly to cruzain, the major lysosomal T. cruzi cysteine protease, involved with infectivity and survival of the parasite in mammalian host cells. In the scope of a project to characterize proteins diferentially expressed during T. cruzi metacyclogenesis, we have determined the crystal structure of chagasin, which is now the first X-ray structure of a chagasin-like cysteine protease inhibitor to be reported. The structure was solved by the SIRAS method and refined at 1.7A resolution and a comparison with the two NMR structures available revealed some differences in the loops involved in binding to cysteine proteases. The highly flexible loop 4 could be entirely modeled and residues 29-33 from loop 2 form a 3(10)-helix structure that may be important to stabilize the loop conformation. Chagasin crystal structure was docked to the highest resolution structure available of cruzain and a model of chagasin-cruzain interaction was analyzed. The knowledge of the chagasin crystal structure may contribute to the elucidation of the molecular mechanism involved in the inhibition of cruzain and other T. cruzi cysteine proteases.

Crystal structure of chagasin, the endogenous cysteine-protease inhibitor from Trypanosoma cruzi.,Figueiredo da Silva AA, de Carvalho Vieira L, Krieger MA, Goldenberg S, Zanchin NI, Guimaraes BG J Struct Biol. 2007 Feb;157(2):416-23. Epub 2006 Aug 17. PMID:17011790<ref>PMID:17011790</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2h7w" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Trycr]]

[[Category: Guimaraes, B G]]

[[Category: Silva, A A.Figueiredo da]]

[[Category: Beta-strand]]

[[Category: Immunoglobulin-fold]]