2ns6

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162

Structural highlights

2ns6 is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

D0VWX2_PSEAI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The plasmid R1162 encodes proteins that enable its conjugative mobilization between bacterial cells. It can transfer between many different species and is one of the most promiscuous of the mobilizable plasmids. The plasmid-encoded protein MobA, which has both nicking and priming activities on single-stranded DNA, is essential for mobilization. The nicking, or relaxase, activity has been localized to the 186 residue N-terminal domain, called minMobA. We present here the 2.1 A X-ray structure of minMobA. The fold is similar to that seen for two other relaxases, TraI and TrwC. The similarity in fold, and action, suggests these enzymes are evolutionary homologs, despite the lack of any significant amino acid similarity. MinMobA has a well- defined target DNA called oriT. The active site metal is observed near Tyr25, which is known to form a phosphotyrosine adduct with the substrate. A model of the oriT substrate complexed with minMobA has been made, based on observed substrate binding to TrwC and TraI. The model is consistent with observations of substrate base specificity, and provides a rationalization for elements of the likely enzyme mechanism.

The structure of the minimal relaxase domain of MobA at 2.1 A resolution.,Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:17157875^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD. The structure of the minimal relaxase domain of MobA at 2.1 A resolution. J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:17157875 doi:10.1016/j.jmb.2006.11.031

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ns6"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162==
-<StructureSection load='2ns6' size='340' side='right' caption='[[2ns6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='2ns6' size='340' side='right'caption='[[2ns6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ns6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NS6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ns6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS6 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mobA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ns6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns6 OCA], [http://pdbe.org/2ns6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ns6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ns6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns6 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns6 OCA], [https://pdbe.org/2ns6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns6 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns6 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/D0VWX2_PSEAI D0VWX2_PSEAI]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns6_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Meyer, R J]]
+[[Category: Large Structures]]
-[[Category: Monzingo, A F]]
+[[Category: Pseudomonas aeruginosa]]
-[[Category: Ozburn, A]]
+[[Category: Meyer RJ]]
-[[Category: Robertus, J D]]
+[[Category: Monzingo AF]]
-[[Category: Xia, S]]
+[[Category: Ozburn A]]
-[[Category: 5-strand antiparallel beta sheet]]
+[[Category: Robertus JD]]
-[[Category: Hydrolase]]
+[[Category: Xia S]]
-[[Category: Metalloenzyme]]
-[[Category: Nickase]]

2ns6

From Proteopedia

Current revision

Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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