2ns7

From Proteopedia

(Difference between revisions)

Current revision

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor

Structural highlights

2ns7 is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TETR2_ECOLX TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A 16-residue peptide, called Tip, induces the tetracycline repressor TetR as efficiently as the antibiotic tetracycline when fused to the N or C terminus of another protein. This is unusual because the majority of in vitro selected peptides, such as Tip, inhibit protein function, and agonist peptides are only rarely identified. We elucidated the atomic mechanism of TetR induction by Tip from crystal structures of TetR in complex with Tip and of free TetR. Peptide induction ultimately results in the same movements of DNA reading heads, but Tip accomplishes this by very different molecular interactions from tetracycline involving important contacts to the TetR surface. As a direct consequence, an alternate pathway of allostery becomes possible that makes ample use of intersubunit interactions. For the first time it is possible to show in atomic detail how a small molecule controlled bacterial transcription factor such as TetR becomes responsive to protein-protein interactions, characteristic of gene transcription regulation in higher organisms.

How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor.,Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA J Mol Biol. 2007 May 4;368(3):780-90. Epub 2007 Feb 22. PMID:17374541^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA. How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor. J Mol Biol. 2007 May 4;368(3):780-90. Epub 2007 Feb 22. PMID:17374541 doi:10.1016/j.jmb.2007.02.030

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ns7"

@@ Line 1: / Line 1: @@
 ==How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor==
-<StructureSection load='2ns7' size='340' side='right' caption='[[2ns7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='2ns7' size='340' side='right'caption='[[2ns7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ns7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NS7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ns7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS7 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2tct|2tct]], [[1qpi|1qpi]], [[2ns8|2ns8]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns7 OCA], [https://pdbe.org/2ns7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns7 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ns7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns7 OCA], [http://pdbe.org/2ns7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ns7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ns7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
+[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns7_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 28: @@
 </div>
 <div class="pdbe-citations 2ns7" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
-[[Category: Berens, C]]
+[[Category: Large Structures]]
-[[Category: Hillen, W]]
+[[Category: Berens C]]
-[[Category: Klotzsche, M]]
+[[Category: Hillen W]]
-[[Category: Luckner, S R]]
+[[Category: Klotzsche M]]
-[[Category: Muller, Y A]]
+[[Category: Luckner SR]]
-[[Category: Helix-turn-helix]]
+[[Category: Muller YA]]
-[[Category: Transcription]]
-[[Category: Transcription regulator]]

2ns7

From Proteopedia

Current revision

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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