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| | ==Structure and Function Studies of Cytochrome P450 158A1 from Streptomyces coelicolor A3(2)== | | ==Structure and Function Studies of Cytochrome P450 158A1 from Streptomyces coelicolor A3(2)== |
| - | <StructureSection load='2nz5' size='340' side='right' caption='[[2nz5]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='2nz5' size='340' side='right'caption='[[2nz5]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2nz5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NZ5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2nz5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZ5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=226:NAPHTHALENE-1,2,4,5,7-PENTOL'>226</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2nza|2nza]], [[1t93|1t93]], [[1se6|1se6]], [[1s1f|1s1f]], [[2d09|2d09]], [[2d0e|2d0e]], [[2dkk|2dkk]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=226:NAPHTHALENE-1,2,4,5,7-PENTOL'>226</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SC8F11.24c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nz5 OCA], [https://pdbe.org/2nz5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nz5 RCSB], [https://www.ebi.ac.uk/pdbsum/2nz5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nz5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nz5 OCA], [http://pdbe.org/2nz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nz5 RCSB], [http://www.ebi.ac.uk/pdbsum/2nz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nz5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/C1581_STRCO C1581_STRCO]] Catalyze oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments which protect the soil bacterium from deleterious effects of UV irradiation (two isomers of biflaviolin and one triflaviolin).<ref>PMID:17614370</ref> | + | [https://www.uniprot.org/uniprot/C1581_STRCO C1581_STRCO] Catalyze oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments which protect the soil bacterium from deleterious effects of UV irradiation (two isomers of biflaviolin and one triflaviolin).<ref>PMID:17614370</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/2nz5_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/2nz5_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 2nz5" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 2nz5" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Strco]] | + | [[Category: Large Structures]] |
| - | [[Category: Kelly, S L]] | + | [[Category: Kelly SL]] |
| - | [[Category: Lamb, D C]] | + | [[Category: Lamb DC]] |
| - | [[Category: Waterman, M R]] | + | [[Category: Waterman MR]] |
| - | [[Category: Zhao, B]] | + | [[Category: Zhao B]] |
| - | [[Category: C-c coupling activity]]
| + | |
| - | [[Category: Cyp158a1]]
| + | |
| - | [[Category: Cytochrome p450 oxidoreductase]]
| + | |
| - | [[Category: Flaviolin]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Streptomyce]]
| + | |
| Structural highlights
Function
C1581_STRCO Catalyze oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments which protect the soil bacterium from deleterious effects of UV irradiation (two isomers of biflaviolin and one triflaviolin).[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradiation (Zhao B. et al. (2005) J. Biol. Chem. 280, 11599-11607). The present studies demonstrate that the subfamily partner CYP158A1, sharing 61% amino acid identity with CYP158A2, can also catalyze the same flaviolin dimerization reactions, but it generates just two of the three isomers of biflaviolin that CYP158A2 produces. Furthermore, the two CYP158A1 products have very different molar ratios compared with the corresponding CYP158A2 products, indicating that each enzyme maintains its own stereo- and regiospecificity. To find an explanation for these differences, three CYP158A1 structures have been solved by X-ray crystallography and have been compared with those for CYP158A2. The structures reveal surprising differences. Particularly, only one flaviolin molecule is present close to the heme iron in CYP158A1, and the second flaviolin molecule binds at the entrance of the putative substrate access channel on the protein distal surface 9 A away. Our work describes two members of the same P450 subfamily, which produce the same products by oxidative C-C coupling yet show very different structural orientations of substrate molecules in the active site.
Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2.,Zhao B, Lamb DC, Lei L, Kelly SL, Yuan H, Hachey DL, Waterman MR Biochemistry. 2007 Jul 31;46(30):8725-33. Epub 2007 Jul 6. PMID:17614370[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhao B, Lamb DC, Lei L, Kelly SL, Yuan H, Hachey DL, Waterman MR. Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2. Biochemistry. 2007 Jul 31;46(30):8725-33. Epub 2007 Jul 6. PMID:17614370 doi:10.1021/bi7006959
- ↑ Zhao B, Lamb DC, Lei L, Kelly SL, Yuan H, Hachey DL, Waterman MR. Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2. Biochemistry. 2007 Jul 31;46(30):8725-33. Epub 2007 Jul 6. PMID:17614370 doi:10.1021/bi7006959
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