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1ywo

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Phospholipase Cgamma1 SH3 in complex with a SLP-76 motif

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Retrieved from "http://52.214.119.220/wiki/index.php/1ywo"

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-[[Image:1ywo.gif|left|200px]]
- {{Structure
+==Phospholipase Cgamma1 SH3 in complex with a SLP-76 motif==
-|PDB= 1ywo |SIZE=350|CAPTION= <scene name='initialview01'>1ywo</scene>, resolution 1.81&Aring;
+<StructureSection load='1ywo' size='340' side='right'caption='[[1ywo]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1ywo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YWO FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoinositide_phospholipase_C Phosphoinositide phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.11 3.1.4.11] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
-|GENE= Plcg1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ywo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ywo OCA], [https://pdbe.org/1ywo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ywo RCSB], [https://www.ebi.ac.uk/pdbsum/1ywo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ywo ProSAT]</span></td></tr>
-|DOMAIN=
+</table>
-|RELATEDENTRY=
+== Function ==
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ywo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ywo OCA], [http://www.ebi.ac.uk/pdbsum/1ywo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ywo RCSB]</span>
+[https://www.uniprot.org/uniprot/PLCG1_RAT PLCG1_RAT] Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration (By similarity).
-}}
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yw/1ywo_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ywo ConSurf].
+<div style="clear:both"></div>
-'''Phospholipase Cgamma1 SH3 in complex with a SLP-76 motif'''
+==See Also==
+*[[Phospholipase C|Phospholipase C]]
+__TOC__
-==Overview==
+</StructureSection>
-The enzyme phospholipase Cgamma1 (PLCgamma1) is essential for T cell signaling and activation. Following T cell receptor ligation, PLCgamma1 interacts through its SH2 and SH3 domains with the adaptors LAT and SLP-76, respectively, to form a multiprotein signaling complex that leads to activation of PLCgamma1 by Syk tyrosine kinases. To identify the binding site for PLCgamma1 in SLP-76, we used isothermal titration calorimetry to measure affinities for the interaction of PLCgamma1-SH3 with a set of overlapping peptides spanning the central proline-rich region of SLP-76. PLCgamma1-SH3 bound with high specificity to the SLP-76 motif 186PPVPPQRP193, which represents the minimal binding site. To understand the basis for selective recognition, we determined the crystal structures of PLCgamma1-SH3 in free form, and bound to a 10-mer peptide containing this site, to resolutions of 1.60 A and 1.81 A, respectively. The structures reveal that several key contacting residues of the SH3 shift toward the SLP-76 peptide upon complex formation, optimizing the fit and strengthening hydrophobic interactions. Selectivity results mainly from strict shape complementarity between protein and peptide, rather than sequence-specific hydrogen bonding. In addition, Pro193 of SLP-76 assists in positioning Arg192 into the compass pocket of PLCgamma1-SH3, which coordinates the compass residue through an unusual aspartate. The PLCgamma1-SH3/SLP-76 structure provides insights into ligand binding by SH3 domains related to PLCgamma1-SH3, as well as into recognition by PLCgamma1 of signaling partners other than SLP-76.
-==About this Structure==
-YWO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWO OCA].
-==Reference==
-Structural basis for recognition of the T cell adaptor protein SLP-76 by the SH3 domain of phospholipase Cgamma1., Deng L, Velikovsky CA, Swaminathan CP, Cho S, Mariuzza RA, J Mol Biol. 2005 Sep 9;352(1):1-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16061254 16061254]
 [[Category: Homo sapiens]]
-[[Category: Phosphoinositide phospholipase C]]
+[[Category: Large Structures]]
-[[Category: Protein complex]]
 [[Category: Rattus norvegicus]]
-[[Category: Cho, S.]]
+[[Category: Cho S]]
-[[Category: Deng, L.]]
+[[Category: Deng L]]
-[[Category: Mariuzza, R A.]]
+[[Category: Mariuzza RA]]
-[[Category: Swaminathan, C P.]]
+[[Category: Swaminathan CP]]
-[[Category: Velikovsky, C A.]]
+[[Category: Velikovsky CA]]
-[[Category: phospholipase c-gamma1]]
-[[Category: sh2 domain-containing leukocyte phosphoprotein of 76 kd]]
-[[Category: sh3]]
-[[Category: slp-76]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:25:18 2008''

1ywo

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Phospholipase Cgamma1 SH3 in complex with a SLP-76 motif

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Evolutionary Conservation

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