|
|
| (13 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1yxn.gif|left|200px]] | |
| | | | |
| - | {{Structure
| + | ==Pseudo-atomic model of a fiberless isometric variant of bacteriophage phi29== |
| - | |PDB= 1yxn |SIZE=350|CAPTION= <scene name='initialview01'>1yxn</scene>
| + | <SX load='1yxn' size='340' side='right' viewer='molstar' caption='[[1yxn]], [[Resolution|resolution]] 7.90Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>
| + | <table><tr><td colspan='2'>[[1yxn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YXN FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.9Å</td></tr> |
| - | |GENE= 8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 Bacillus phage phi29])
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yxn OCA], [https://pdbe.org/1yxn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yxn RCSB], [https://www.ebi.ac.uk/pdbsum/1yxn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yxn ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=[[1ohg|1OHG]], [[1f00|1F00]]
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yxn OCA], [http://www.ebi.ac.uk/pdbsum/1yxn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yxn RCSB]</span>
| + | </SX> |
| - | }}
| + | [[Category: Bacillus virus phi29]] |
| - | | + | [[Category: Large Structures]] |
| - | '''Pseudo-atomic model of a fiberless isometric variant of bacteriophage phi29'''
| + | [[Category: Anderson DL]] |
| - | | + | [[Category: Chipman PR]] |
| - | | + | [[Category: Choi KH]] |
| - | ==Overview==
| + | [[Category: Koti JS]] |
| - | Bacteriophage phi29 is one of the smallest and simplest known dsDNA phages, making it amenable to structural investigations. The three-dimensional structure of a fiberless, isometric variant has been determined to 7.9 A resolution by cryo-electron microscopy (cryo-EM), allowing the identification of alpha helices and beta sheets. Their arrangement indicates that the folds of the phi29 and bacteriophage HK97 capsid proteins are similar except for an additional immunoglobulin-like domain of the phi29 protein. An atomic model that incorporates these two domains fits well into the cryo-EM density of the T = 3, fiberless isometric phi29 particle, and cryo-EM structures of fibered isometric and fiberless prolate prohead phi29 particles at resolutions of 8.7 A and 12.7 A, respectively. Thus, phi29 joins the growing number of phages that utilize the HK97 capsid structure, suggesting that this protein fold may be as prevalent in capsids of dsDNA phages as the jelly roll fold is in eukaryotic viruses.
| + | [[Category: Morais MC]] |
| - | | + | [[Category: Rossmann MG]] |
| - | ==About this Structure==
| + | |
| - | 1YXN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YXN OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Conservation of the capsid structure in tailed dsDNA bacteriophages: the pseudoatomic structure of phi29., Morais MC, Choi KH, Koti JS, Chipman PR, Anderson DL, Rossmann MG, Mol Cell. 2005 Apr 15;18(2):149-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15837419 15837419]
| + | |
| - | [[Category: Bacillus phage phi29]] | + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Anderson, D L.]] | + | |
| - | [[Category: Chipman, P R.]] | + | |
| - | [[Category: Choi, K H.]] | + | |
| - | [[Category: Koti, J S.]] | + | |
| - | [[Category: Morais, M C.]] | + | |
| - | [[Category: Rossmann, M G.]] | + | |
| - | [[Category: bacterial immuno-globulin]]
| + | |
| - | [[Category: big2]]
| + | |
| - | [[Category: capsid]]
| + | |
| - | [[Category: hk97 fold]]
| + | |
| - | [[Category: icosahedral virus]]
| + | |
| - | [[Category: icosahedral virus capsid]]
| + | |
| - | [[Category: phage]]
| + | |
| - | [[Category: phi29]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:26:03 2008''
| + | |
