|
|
| (4 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution structure of a toxin (GsMTx2) from the tarantula, Grammostola spatulata, which inhibits mechanosensitive ion channels== | | ==Solution structure of a toxin (GsMTx2) from the tarantula, Grammostola spatulata, which inhibits mechanosensitive ion channels== |
| - | <StructureSection load='1lup' size='340' side='right' caption='[[1lup]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1lup' size='340' side='right'caption='[[1lup]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1lup]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilean_red-back Chilean red-back]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LUP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1lup]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Grammostola_rosea Grammostola rosea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LUP FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lqr|1lqr]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lup OCA], [http://pdbe.org/1lup PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lup RCSB], [http://www.ebi.ac.uk/pdbsum/1lup PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lup ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lup OCA], [https://pdbe.org/1lup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lup RCSB], [https://www.ebi.ac.uk/pdbsum/1lup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lup ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/MTX2_GRARO MTX2_GRARO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chilean red-back]] | + | [[Category: Grammostola rosea]] |
| - | [[Category: Gottlieb, P]] | + | [[Category: Large Structures]] |
| - | [[Category: McFeeters, R]] | + | [[Category: Gottlieb P]] |
| - | [[Category: Oswald, R E]] | + | [[Category: McFeeters R]] |
| - | [[Category: Sachs, F]] | + | [[Category: Oswald RE]] |
| - | [[Category: Suchyna, T M]] | + | [[Category: Sachs F]] |
| - | [[Category: Beta-sheet]]
| + | [[Category: Suchyna TM]] |
| - | [[Category: Inhibitor cysteine knot]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
MTX2_GRARO
Publication Abstract from PubMed
Mechanosensitive channels (MSCs) play key roles in sensory processing and have been implicated as primary transducers for a variety of cellular responses ranging from osmosensing to gene expression. This paper presents the first structures of any kind known to interact specifically with MSCs. GsMTx-4 and GsMtx-2 are inhibitor cysteine knot peptides isolated from venom of the tarantula, Grammostola spatulata (Suchyna, T. M., Johnson, J. H., Hamer, K., Leykam, J. F., Gage, D. A., Clemo, H. F., Baumgarten, C. M., and Sachs, F. (2000) J. Gen. Physiol. 115, 583-598). Inhibition of cationic MSCs by the higher affinity GsMtx-4 (K(D) approximately 500 nm) reduced cell size in swollen and hypertrophic heart cells, swelling-activated currents in astrocytes, and stretch-induced arrhythmias in the heart. Despite the relatively low affinity, no cross-reactivity has been found with other channels. Using two-dimensional NMR spectroscopy, we determined the solution structure of GsMTx-4 and a lower affinity (GsMTx-2; K(D) approximately 6 microm) peptide from the same venom. The dominant feature of the two structures is a hydrophobic patch, utilizing most of the aromatic residues and surrounded with charged residues. The spatial arrangement of charged residues that are unique to GsMTx-4 and GsMTx-2 may underlie the selectivity of these peptides.
Solution structure of peptide toxins that block mechanosensitive ion channels.,Oswald RE, Suchyna TM, McFeeters R, Gottlieb P, Sachs F J Biol Chem. 2002 Sep 13;277(37):34443-50. Epub 2002 Jun 24. PMID:12082099[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Oswald RE, Suchyna TM, McFeeters R, Gottlieb P, Sachs F. Solution structure of peptide toxins that block mechanosensitive ion channels. J Biol Chem. 2002 Sep 13;277(37):34443-50. Epub 2002 Jun 24. PMID:12082099 doi:10.1074/jbc.M202715200
|
