1lqx

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of V45E mutant of cytochrome b5

Structural highlights

1lqx is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CYB5_BOVIN Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Val45 is a highly conserved residue and a component of the heme-pocket wall of cytochrome b(5). The crystal structures of cytochrome b(5) mutants V45E and V45Y have been determined at high resolution. Their overall structures were very similar to that of the wild-type protein. However, Val45 of the wild-type protein points towards the heme, but the large side chains of both Glu45 and Tyr45 of the mutants point towards the solvent. A channel is thus opened and the hydrophobicity of the heme pocket is decreased. The rotation of the porphyrin ring and the conformational change of the axial ligand His39 in the V45Y mutant indicate that the microenvironment of the heme is disturbed because of the mutation. The binding constants and the electron-transfer rates between cytochrome b(5) and cytochrome c decrease owing to the mutation, which can be accounted for by molecular modeling: the inter-iron distances increase in order to eliminate the unreasonably close contacts resulting from the large volumes of the mutated side chains. The influence of the mutations on the redox potentials and protein stability is also discussed. The structures of seven mutants of cytochrome b(5) are compared with each other and the effects of these mutations on the protein properties and functions are summarized.

Structures of V45E and V45Y mutants and structure comparison of a variety of cytochrome b5 mutants.,Gan JH, Wu J, Wang ZQ, Wang YH, Huang ZX, Xia ZX Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1298-306. Epub 2002, Jul 20. PMID:12136141^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gan JH, Wu J, Wang ZQ, Wang YH, Huang ZX, Xia ZX. Structures of V45E and V45Y mutants and structure comparison of a variety of cytochrome b5 mutants. Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1298-306. Epub 2002, Jul 20. PMID:12136141

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lqx"

@@ Line 1: / Line 1: @@
 ==Crystal structure of V45E mutant of cytochrome b5==
-<StructureSection load='1lqx' size='340' side='right' caption='[[1lqx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='1lqx' size='340' side='right'caption='[[1lqx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1lqx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LQX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1lqx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ehb|1ehb]], [[1es1|1es1]], [[1lr6|1lr6]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqx OCA], [http://pdbe.org/1lqx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lqx RCSB], [http://www.ebi.ac.uk/pdbsum/1lqx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqx ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqx OCA], [https://pdbe.org/1lqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqx RCSB], [https://www.ebi.ac.uk/pdbsum/1lqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqx ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CYB5_BOVIN CYB5_BOVIN]] Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
+[https://www.uniprot.org/uniprot/CYB5_BOVIN CYB5_BOVIN] Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqx_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 29: @@
 </div>
 <div class="pdbe-citations 1lqx" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cytochrome b5 3D structures|Cytochrome b5 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bovin]]
+[[Category: Bos taurus]]
-[[Category: Gan, J H]]
+[[Category: Large Structures]]
-[[Category: Huang, Z X]]
+[[Category: Gan J-H]]
-[[Category: Wang, Y H]]
+[[Category: Huang Z-X]]
-[[Category: Wang, Z Q]]
+[[Category: Wang Y-H]]
-[[Category: Wu, J]]
+[[Category: Wang Z-Q]]
-[[Category: Xia, Z X]]
+[[Category: Wu J]]
-[[Category: Cytochrome b5]]
+[[Category: Xia Z-X]]
-[[Category: Electron transport]]
-[[Category: Mutant v45e]]
-[[Category: Trypsin-cleaved fragment]]

1lqx

From Proteopedia

Current revision

Crystal structure of V45E mutant of cytochrome b5

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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