1z26

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:02, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1z26.gif|left|200px]]
 
-
{{Structure
+
==Structure of Pyrococcus furiosus Argonaute with bound tungstate==
-
|PDB= 1z26 |SIZE=350|CAPTION= <scene name='initialview01'>1z26</scene>, resolution 2.50&Aring;
+
<StructureSection load='1z26' size='340' side='right'caption='[[1z26]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene>
+
<table><tr><td colspan='2'>[[1z26]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z26 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z26 OCA], [https://pdbe.org/1z26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z26 RCSB], [https://www.ebi.ac.uk/pdbsum/1z26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z26 ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1u04|1U04]], [[1z25|1Z25]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z26 OCA], [http://www.ebi.ac.uk/pdbsum/1z26 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z26 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q8U3D2_PYRFU Q8U3D2_PYRFU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/1z26_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z26 ConSurf].
 +
<div style="clear:both"></div>
-
'''Structure of Pyrococcus furiosus Argonaute with bound tungstate'''
+
==See Also==
-
 
+
*[[Argonaute 3D structures|Argonaute 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human Argonaute2 can combine with a small interfering RNA (siRNA) to form minimal RISC that accurately cleaves substrate RNAs. Recombinant RISC shows many of the properties of RISC purified from human or Drosophila melanogaster cells but also has surprising features. It shows no stimulation by ATP, suggesting that factors promoting product release are missing from the recombinant enzyme. The active site is made up of a unique Asp-Asp-His (DDH) motif. In the RISC reconstitution system, the siRNA 5' phosphate is important for the stability and the fidelity of the complex but is not essential for the creation of an active enzyme. These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1Z26 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z26 OCA].
+
-
 
+
-
==Reference==
+
-
Purified Argonaute2 and an siRNA form recombinant human RISC., Rivas FV, Tolia NH, Song JJ, Aragon JP, Liu J, Hannon GJ, Joshua-Tor L, Nat Struct Mol Biol. 2005 Apr;12(4):340-9. Epub 2005 Mar 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15800637 15800637]
+
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
-
[[Category: Single protein]]
+
[[Category: Aragon JP]]
-
[[Category: Aragon, J P.]]
+
[[Category: Hannon GJ]]
-
[[Category: Hannon, G J.]]
+
[[Category: Joshua-Tor L]]
-
[[Category: Joshua-Tor, L.]]
+
[[Category: Liu J]]
-
[[Category: Liu, J.]]
+
[[Category: Rivas FV]]
-
[[Category: Rivas, F V.]]
+
[[Category: Song JJ]]
-
[[Category: Song, J J.]]
+
[[Category: Tolia NH]]
-
[[Category: Tolia, N H.]]
+
-
[[Category: argonaute]]
+
-
[[Category: rnai]]
+
-
[[Category: tungstate]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:28:00 2008''
+

Current revision

Structure of Pyrococcus furiosus Argonaute with bound tungstate

PDB ID 1z26

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z26"
Personal tools