Ketol-acid reductoisomerase
From Proteopedia
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<StructureSection load='3ulk' size='340' side='right' caption='E. coli class II ketol-acid reductoisomerase complex with NADPH, sulfate and Mg+2 ion (PDB code [[3ulk]]) ' scene=''> | <StructureSection load='3ulk' size='340' side='right' caption='E. coli class II ketol-acid reductoisomerase complex with NADPH, sulfate and Mg+2 ion (PDB code [[3ulk]]) ' scene=''> | ||
== Function == | == Function == | ||
| - | '''Ketol-acid reductoisomerase''' (KARI) is a bifunctional enzyme which catalizes two steps in the biosynthesis of branched-chain amino acids. KARI catalyzes the isomerization of alkyls and the NADPH-dependent reduction of a 2-ketoacid<ref>PMID:15654896</ref>. There are two forms of KARI. A short form – '''Class I''' – found in fungi and most bacteria and a long form – '''Class II''' – found in plants. | + | '''Ketol-acid reductoisomerase''' (KARI) is a bifunctional enzyme which catalizes two steps in the biosynthesis of branched-chain amino acids. KARI catalyzes the isomerization of alkyls and the NADPH-dependent reduction of a 2-ketoacid<ref>PMID:15654896</ref>. There are two forms of KARI. A short form – '''Class I''' – found in fungi and most bacteria and a long form. For the active site see [[Arnold lab: coenzyme specificity]] – '''Class II''' – found in plants. |
| + | == 3D Structures of Ketol-acid reductoisomerase == | ||
| + | [[Ketol-acid reductoisomerase 3D structures]] | ||
</StructureSection> | </StructureSection> | ||
| - | == 3D Structures of Ketol-acid reductoisomerase == | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *KARI class I | ||
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| - | **[[1np3]] – KARI – ''Pseudomonas aeruginosa''<br /> | ||
| - | **[[4xeh]] – IaKARI – ''Ignisphaera aggregans''<br /> | ||
| - | **[[4xiy]] – KARI + Fe + Mg – ''Azotobacter vinelandii''<br /> | ||
| - | **[[4ypo]] – KARI + Mg – ''Mycobcterium tuberculosis''<br /> | ||
| - | **[[4kqw]] – SeKARI + NADPH + Mg – ''Slackia exigua''<br /> | ||
| - | **[[4tsk]] – KARI + NADPH + Mg – ''Alicyclobacillus acidocaldarius''<br /> | ||
| - | **[[4xdy]] – KARI + NADPH + Mg – archea<br /> | ||
| - | **[[4xdz]] – IaKARI + NADPH + Mg <br /> | ||
| - | **[[4kqx]] – SeKARI + NAD + Mg + hydroxyl-isopropyloxamate <br /> | ||
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| - | *KARI class II | ||
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| - | **[[1yrl]] – EcKARI – ''Escherichia coli''<br /> | ||
| - | **[[3ulk]] – EcKARI + NADPH + Mg<br /> | ||
| - | **[[3fr7]] – rKARI + Mg - rice<br /> | ||
| - | **[[3fr8]] – rKARI + NADPH + Mg<br /> | ||
| - | **[[1yve]] – sKARI + NADPH + hydroxyl-isopropyloxamate - spinach<br /> | ||
| - | **[[1qmg]] – sKARI + Mn + dihydroxy-valerianate + AMP-diphosphoribose<br /> | ||
| - | **[[5e4r]] – KARI + NADPH + Mg – ''Ignisphaera aggregans''<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Tyagi R, Lee YT, Guddat LW, Duggleby RG. Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site. FEBS J. 2005 Jan;272(2):593-602. PMID:15654896 doi:http://dx.doi.org/10.1111/j.1742-4658.2004.04506.x
