1z4x

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[[Image:1z4x.gif|left|200px]]
 
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{{Structure
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==Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand Sialyllactose (soaked with Sialyllactose, pH8.0)==
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|PDB= 1z4x |SIZE=350|CAPTION= <scene name='initialview01'>1z4x</scene>, resolution 2.500&Aring;
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<StructureSection load='1z4x' size='340' side='right'caption='[[1z4x]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SLT:5-(ACETYLAMINO)-3,5-DIDEOXYNON-2-ULOPYRANONOSYL-(2-&#62;3)-BETA-D-LYXO-HEXOPYRANOSYL-(1-&#62;4)HEXOPYRANOSE'>SLT</scene>
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<table><tr><td colspan='2'>[[1z4x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthorubulavirus_5 Mammalian orthorubulavirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z4X FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= HN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11207 Simian virus 5])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900025:3-sialyl-alpha-lactose'>PRD_900025</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z4x OCA], [https://pdbe.org/1z4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z4x RCSB], [https://www.ebi.ac.uk/pdbsum/1z4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z4x ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1z4v|1Z4V]], [[1z4w|1Z4W]], [[1z4y|1Z4Y]], [[1z4z|1Z4Z]], [[1z50|1Z50]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z4x OCA], [http://www.ebi.ac.uk/pdbsum/1z4x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z4x RCSB]</span>
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== Function ==
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[https://www.uniprot.org/uniprot/HN_PIV5 HN_PIV5] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.
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== Evolutionary Conservation ==
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'''Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand Sialyllactose (soaked with Sialyllactose, pH8.0)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/1z4x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z4x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. The structural basis for these diverse functions remains to be fully understood. We report the crystal structures of the parainfluenza virus 5 (SV5) HN and its complexes with sialic acid, the inhibitor DANA, and the receptor sialyllactose. SV5 HN shares common structural features with HN of Newcastle disease virus (NDV) and human parainfluenza 3 (HPIV3), but unlike the previously determined HN structures, the SV5 HN forms a tetramer in solution, which is thought to be the physiological oligomer. The sialyllactose complex reveals intact receptor within the active site, but no major conformational changes in the protein. The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells.
The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. The structural basis for these diverse functions remains to be fully understood. We report the crystal structures of the parainfluenza virus 5 (SV5) HN and its complexes with sialic acid, the inhibitor DANA, and the receptor sialyllactose. SV5 HN shares common structural features with HN of Newcastle disease virus (NDV) and human parainfluenza 3 (HPIV3), but unlike the previously determined HN structures, the SV5 HN forms a tetramer in solution, which is thought to be the physiological oligomer. The sialyllactose complex reveals intact receptor within the active site, but no major conformational changes in the protein. The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells.
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==About this Structure==
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Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose.,Yuan P, Thompson TB, Wurzburg BA, Paterson RG, Lamb RA, Jardetzky TS Structure. 2005 May;13(5):803-15. PMID:15893670<ref>PMID:15893670</ref>
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1Z4X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_5 Simian virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z4X OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose., Yuan P, Thompson TB, Wurzburg BA, Paterson RG, Lamb RA, Jardetzky TS, Structure. 2005 May;13(5):803-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15893670 15893670]
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</div>
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[[Category: Exo-alpha-sialidase]]
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<div class="pdbe-citations 1z4x" style="background-color:#fffaf0;"></div>
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[[Category: Simian virus 5]]
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[[Category: Single protein]]
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[[Category: Jardetzky, T S.]]
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[[Category: Lamb, R A.]]
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[[Category: Paterson, R G.]]
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[[Category: Thompson, T B.]]
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[[Category: Wurzburg, B A.]]
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[[Category: Yuan, P.]]
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[[Category: hemagglutinin,neuraminidase,fusion,tetramer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:07 2008''
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==See Also==
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*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mammalian orthorubulavirus 5]]
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[[Category: Jardetzky TS]]
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[[Category: Lamb RA]]
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[[Category: Paterson RG]]
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[[Category: Thompson TB]]
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[[Category: Wurzburg BA]]
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[[Category: Yuan P]]

Current revision

Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand Sialyllactose (soaked with Sialyllactose, pH8.0)

PDB ID 1z4x

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