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| | ==Structure of the DNA-binding domain of LUX ARRHYTHMO== | | ==Structure of the DNA-binding domain of LUX ARRHYTHMO== |
| - | <StructureSection load='5lxu' size='340' side='right' caption='[[5lxu]], [[Resolution|resolution]] 2.14Å' scene=''> | + | <StructureSection load='5lxu' size='340' side='right'caption='[[5lxu]], [[Resolution|resolution]] 2.14Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lxu]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LXU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lxu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LXU FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.14Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxu OCA], [http://pdbe.org/5lxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxu RCSB], [http://www.ebi.ac.uk/pdbsum/5lxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxu ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxu OCA], [https://pdbe.org/5lxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lxu RCSB], [https://www.ebi.ac.uk/pdbsum/5lxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PCL1_ARATH PCL1_ARATH]] Transcription factor that is essential for the generation of the circadian clock oscillation. Is necessary for activation of CCA1 and LHY expression. Is coregulated with TOC1 and seems to be repressed by CCA1 and LHY by direct binding of these proteins to the evening element in the LUX promoter. Directly regulates the expression of PRR9, a major component of the morning transcriptional feedback circuit, by binding specific sites on PRR9 promoter. Binds to its own promoter, inducing a negative auto-regulatory feedback loop within the core clock. Binds to ELF3 and associates with ELF4 in a diurnal complex which is required for the expression of the growth-promoting transcription factors PIF4 and PIF5 and subsequent hypocotyl growth in the early evening.<ref>PMID:16006522</ref> <ref>PMID:16164597</ref> <ref>PMID:21236673</ref> <ref>PMID:21753751</ref> <ref>PMID:22311777</ref> | + | [https://www.uniprot.org/uniprot/PCL1_ARATH PCL1_ARATH] Transcription factor that is essential for the generation of the circadian clock oscillation. Is necessary for activation of CCA1 and LHY expression. Is coregulated with TOC1 and seems to be repressed by CCA1 and LHY by direct binding of these proteins to the evening element in the LUX promoter. Directly regulates the expression of PRR9, a major component of the morning transcriptional feedback circuit, by binding specific sites on PRR9 promoter. Binds to its own promoter, inducing a negative auto-regulatory feedback loop within the core clock. Binds to ELF3 and associates with ELF4 in a diurnal complex which is required for the expression of the growth-promoting transcription factors PIF4 and PIF5 and subsequent hypocotyl growth in the early evening.<ref>PMID:16006522</ref> <ref>PMID:16164597</ref> <ref>PMID:21236673</ref> <ref>PMID:21753751</ref> <ref>PMID:22311777</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Nanao, M H]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Zubieta, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Nanao MH]] |
| - | [[Category: Dna-binding]] | + | [[Category: Zubieta C]] |
| - | [[Category: Myb domain]]
| + | |
| - | [[Category: Three helix bundle]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| Structural highlights
Function
PCL1_ARATH Transcription factor that is essential for the generation of the circadian clock oscillation. Is necessary for activation of CCA1 and LHY expression. Is coregulated with TOC1 and seems to be repressed by CCA1 and LHY by direct binding of these proteins to the evening element in the LUX promoter. Directly regulates the expression of PRR9, a major component of the morning transcriptional feedback circuit, by binding specific sites on PRR9 promoter. Binds to its own promoter, inducing a negative auto-regulatory feedback loop within the core clock. Binds to ELF3 and associates with ELF4 in a diurnal complex which is required for the expression of the growth-promoting transcription factors PIF4 and PIF5 and subsequent hypocotyl growth in the early evening.[1] [2] [3] [4] [5]
References
- ↑ Hazen SP, Schultz TF, Pruneda-Paz JL, Borevitz JO, Ecker JR, Kay SA. LUX ARRHYTHMO encodes a Myb domain protein essential for circadian rhythms. Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10387-92. Epub 2005 Jul 8. PMID:16006522 doi:http://dx.doi.org/10.1073/pnas.0503029102
- ↑ Onai K, Ishiura M. PHYTOCLOCK 1 encoding a novel GARP protein essential for the Arabidopsis circadian clock. Genes Cells. 2005 Oct;10(10):963-72. PMID:16164597 doi:http://dx.doi.org/10.1111/j.1365-2443.2005.00892.x
- ↑ Helfer A, Nusinow DA, Chow BY, Gehrke AR, Bulyk ML, Kay SA. LUX ARRHYTHMO encodes a nighttime repressor of circadian gene expression in the Arabidopsis core clock. Curr Biol. 2011 Jan 25;21(2):126-33. doi: 10.1016/j.cub.2010.12.021. Epub 2011, Jan 13. PMID:21236673 doi:http://dx.doi.org/10.1016/j.cub.2010.12.021
- ↑ Nusinow DA, Helfer A, Hamilton EE, King JJ, Imaizumi T, Schultz TF, Farre EM, Kay SA. The ELF4-ELF3-LUX complex links the circadian clock to diurnal control of hypocotyl growth. Nature. 2011 Jul 13;475(7356):398-402. doi: 10.1038/nature10182. PMID:21753751 doi:http://dx.doi.org/10.1038/nature10182
- ↑ Sellaro R, Pacin M, Casal JJ. Diurnal dependence of growth responses to shade in Arabidopsis: role of hormone, clock, and light signaling. Mol Plant. 2012 May;5(3):619-28. doi: 10.1093/mp/ssr122. Epub 2012 Feb 6. PMID:22311777 doi:http://dx.doi.org/10.1093/mp/ssr122
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