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| - | [[Image:1z96.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of the Mud1 UBA domain== |
| - | |PDB= 1z96 |SIZE=350|CAPTION= <scene name='initialview01'>1z96</scene>, resolution 1.80Å
| + | <StructureSection load='1z96' size='340' side='right'caption='[[1z96]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[1z96]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z96 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | |GENE= mud1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z96 OCA], [https://pdbe.org/1z96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z96 RCSB], [https://www.ebi.ac.uk/pdbsum/1z96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z96 ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z96 OCA], [http://www.ebi.ac.uk/pdbsum/1z96 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z96 RCSB]</span>
| + | [https://www.uniprot.org/uniprot/MUD1_SCHPO MUD1_SCHPO] |
| - | }}
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | '''Crystal structure of the Mud1 UBA domain'''
| + | [[Category: Large Structures]] |
| - | | + | |
| - | | + | |
| - | ==Overview==
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| - | The ubiquitin-pathway associated (UBA) domain is a 40-residue polyubiquitin-binding motif. The Schizosaccharomyces pombe protein Mud1 is an ortholog of the Saccharomyces cerevisiae DNA-damage response protein Ddi1 and binds to K48-linked polyubiquitin through its UBA domain. We have solved the crystal structure of Mud1 UBA at 1.8 angstroms resolution, revealing a canonical three-helical UBA fold. We have probed the interactions of this domain using mutagenesis, surface plasmon resonance, NMR and analytical ultracentrifugation. We show that the ubiquitin-binding surface of Mud1 UBA extends beyond previously recognized motifs and can be functionally dissected into primary and secondary ubiquitin-binding sites. Mutation of Phe330 to alanine, a residue exposed between helices 2 and 3, significantly reduces the affinity of the Mud1 UBA domain for K48-linked polyubiquitin, despite leaving the primary binding surface functionally intact. Moreover, K48-linked diubiquitin binds a single Mud1 UBA domain even in the presence of excess UBA. We therefore propose a mechanism for the recognition of K48-linked polyubiquitin chains by Mud1 in which diubiquitin units are specifically recognized by a single UBA domain.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1Z96 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain., Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA, EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16138082 16138082]
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| | [[Category: Schizosaccharomyces pombe]] | | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: Single protein]]
| + | [[Category: Brown NR]] |
| - | [[Category: Brown, N R.]] | + | [[Category: Campbell ID]] |
| - | [[Category: Campbell, I D.]] | + | [[Category: Endicott JA]] |
| - | [[Category: Endicott, J A.]] | + | [[Category: Gordon C]] |
| - | [[Category: Gordon, C.]] | + | [[Category: Johnson LN]] |
| - | [[Category: Johnson, L N.]] | + | [[Category: Lowe ED]] |
| - | [[Category: Lowe, E D.]] | + | [[Category: Noble MEM]] |
| - | [[Category: Noble, M E.M.]] | + | [[Category: Trempe J-F]] |
| - | [[Category: Trempe, J F.]] | + | |
| - | [[Category: three-helix bundle]]
| + | |
| - | [[Category: uba]]
| + | |
| - | [[Category: ubiquitin]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:32:25 2008''
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