We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1z9l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (00:43, 21 November 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1z9l.gif|left|200px]]
 
-
{{Structure
+
==1.7 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain==
-
|PDB= 1z9l |SIZE=350|CAPTION= <scene name='initialview01'>1z9l</scene>, resolution 1.70&Aring;
+
<StructureSection load='1z9l' size='340' side='right'caption='[[1z9l]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
+
<table><tr><td colspan='2'>[[1z9l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z9L FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
|GENE= Vapa, Vap33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z9l OCA], [https://pdbe.org/1z9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z9l RCSB], [https://www.ebi.ac.uk/pdbsum/1z9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z9l ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1z9o|1Z9O]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z9l OCA], [http://www.ebi.ac.uk/pdbsum/1z9l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z9l RCSB]</span>
+
== Evolutionary Conservation ==
-
}}
+
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z9/1z9l_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z9l ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.
-
'''1.7 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain'''
+
Structural basis of FFAT motif-mediated ER targeting.,Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT Structure. 2005 Jul;13(7):1035-45. PMID:16004875<ref>PMID:16004875</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1z9l" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.
+
*[[Vesicle-associated membrane protein 3D structures|Vesicle-associated membrane protein 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1Z9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9L OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
[[Category: Large Structures]]
-
Structural basis of FFAT motif-mediated ER targeting., Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT, Structure. 2005 Jul;13(7):1035-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16004875 16004875]
+
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Single protein]]
+
[[Category: Brickner JH]]
-
[[Category: Brickner, J H.]]
+
[[Category: Brunger AT]]
-
[[Category: Brunger, A T.]]
+
[[Category: Fenn TD]]
-
[[Category: Fenn, T D.]]
+
[[Category: Kaiser SE]]
-
[[Category: Kaiser, S E.]]
+
[[Category: Reilein AR]]
-
[[Category: Reilein, A R.]]
+
[[Category: Walter P]]
-
[[Category: Walter, P.]]
+
-
[[Category: cytoplasmic domain]]
+
-
[[Category: vap-a]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:32:38 2008''
+

Current revision

1.7 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain

PDB ID 1z9l

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z9l"
Personal tools