3b6a

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin

Structural highlights

3b6a is a 8 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.05Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q53901_STRCH

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is exported from the cell by the ActA efflux pump. actA is divergently transcribed from actR, which encodes a TetR-like transcriptional repressor. We showed previously that ActR represses transcription by binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can cause ActR to dissociate from its operator, leading to derepression. This suggests that ActR may mediate timely self-resistance to an endogenously produced antibiotic by responding to one of its biosynthetic precursors. Here, we report the structural basis for this precursor-mediated derepression with crystal structures of homodimeric ActR by itself and in complex with either actinorhodin or the actinorhodin biosynthetic intermediate (S)-DNPA [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti c acid]. The ligand-binding tunnel in each ActR monomer has a striking hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that accommodate either one hexacyclic actinorhodin molecule or two back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals the strongest structural evidence to date that TetR-mediated antibiotic resistance may have been acquired from an antibiotic-producer organism.

Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA.,Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR J Mol Biol. 2008 Mar 7;376(5):1377-87. Epub 2008 Jan 4. PMID:18207163^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR. Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA. J Mol Biol. 2008 Mar 7;376(5):1377-87. Epub 2008 Jan 4. PMID:18207163 doi:10.1016/j.jmb.2007.12.061

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3b6a"

Categories: Large Structures | Streptomyces coelicolor | Junop MS | Willems AR

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin==
-<StructureSection load='3b6a' size='340' side='right' caption='[[3b6a]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
+<StructureSection load='3b6a' size='340' side='right'caption='[[3b6a]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3b6a]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B6A FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2opt|2opt]], [[3b6c|3b6c]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZCT:2,2-[(1R,1R,3S,3S)-6,6,9,9-TETRAHYDROXY-1,1-DIMETHYL-5,5,10,10-TETRAOXO-3,3,4,4,5,5,10,10-OCTAHYDRO-1H,1H-8,8-BIBENZO[G]ISOCHROMENE-3,3-DIYL]DIACETIC+ACID'>ZCT</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">actR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [https://pdbe.org/3b6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [https://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6a OCA], [http://pdbe.org/3b6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b6a RCSB], [http://www.ebi.ac.uk/pdbsum/3b6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6a ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q53901_STRCH Q53901_STRCH]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b6a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b6a_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 3b6a" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Junop, M S]]
+[[Category: Large Structures]]
-[[Category: Willems, A R]]
+[[Category: Streptomyces coelicolor]]
-[[Category: Actinorhodin]]
+[[Category: Junop MS]]
-[[Category: Dna-binding protein]]
+[[Category: Willems AR]]
-[[Category: Ligand]]
-[[Category: Tetr family]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]
-[[Category: Transcriptional repressor]]

3b6a

From Proteopedia

Current revision

Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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