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| | ==Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164== | | ==Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164== |
| - | <StructureSection load='3v61' size='340' side='right' caption='[[3v61]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='3v61' size='340' side='right'caption='[[3v61]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3v61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V61 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3v61]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V61 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=NEQ:N-ETHYLMALEIMIDE'>NEQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3v60|3v60]], [[3v62|3v62]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=NEQ:N-ETHYLMALEIMIDE'>NEQ</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D9719.15, SMT3, YDR510W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), POL30, YBR0811, YBR088C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v61 OCA], [https://pdbe.org/3v61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v61 RCSB], [https://www.ebi.ac.uk/pdbsum/3v61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v61 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v61 OCA], [http://pdbe.org/3v61 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v61 RCSB], [http://www.ebi.ac.uk/pdbsum/3v61 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v61 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SMT3_YEAST SMT3_YEAST]] Not known; suppressor of MIF2 mutations. [[http://www.uniprot.org/uniprot/PCNA_YEAST PCNA_YEAST]] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.<ref>PMID:11545742</ref> <ref>PMID:12226657</ref> | + | [https://www.uniprot.org/uniprot/SMT3_YEAST SMT3_YEAST] Not known; suppressor of MIF2 mutations. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Ubiquitin (Ub) and ubiquitin-like (Ubl) modifiers such as SUMO (also known as Smt3 in Saccharomyces cerevisiae) mediate signal transduction through post-translational modification of substrate proteins in pathways that control differentiation, apoptosis and the cell cycle, and responses to stress such as the DNA damage response. In yeast, the proliferating cell nuclear antigen PCNA (also known as Pol30) is modified by ubiquitin in response to DNA damage and by SUMO during S phase. Whereas Ub-PCNA can signal for recruitment of translesion DNA polymerases, SUMO-PCNA signals for recruitment of the anti-recombinogenic DNA helicase Srs2. It remains unclear how receptors such as Srs2 specifically recognize substrates after conjugation to Ub and Ubls. Here we show, through structural, biochemical and functional studies, that the Srs2 carboxy-terminal domain harbours tandem receptor motifs that interact independently with PCNA and SUMO and that both motifs are required to recognize SUMO-PCNA specifically. The mechanism presented is pertinent to understanding how other receptors specifically recognize Ub- and Ubl-modified substrates to facilitate signal transduction.
| + | |
| | | | |
| - | Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2.,Armstrong AA, Mohideen F, Lima CD Nature. 2012 Feb 29;483(7387):59-63. doi: 10.1038/nature10883. PMID:22382979<ref>PMID:22382979</ref>
| + | ==See Also== |
| - | | + | *[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | *[[SUMO 3D Structures|SUMO 3D Structures]] |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3v61" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Armstrong, A A]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Lima, C D]] | + | [[Category: Armstrong AA]] |
| - | [[Category: Mohideen, F]] | + | [[Category: Lima CD]] |
| - | [[Category: Dna damage response]] | + | [[Category: Mohideen F]] |
| - | [[Category: Dna replication]]
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| - | [[Category: Nem modification on pcna cys22 and cys81]]
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| - | [[Category: Nuclear]]
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| - | [[Category: Post-translational modification]]
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| - | [[Category: Protein binding-dna binding protein complex]]
| + | |
| - | [[Category: Srs2]]
| + | |
| - | [[Category: Ubiquitin-like protein pcna]]
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