1n89

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of a liganded type 2 wheat non-specific Lipid Transfer Protein

Structural highlights

1n89 is a 1 chain structure with sequence from Triticum turgidum subsp. durum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 9 models
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NLT2G_WHEAT Transfer lipids across membranes. May play a role in plant defense or in the biosynthesis of cuticle layers.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The refined structure of a wheat type 2 nonspecific lipid transfer protein (ns-LTP2) liganded with l-alpha-palmitoylphosphatidylglycerol has been determined by NMR. The (15)N-labeled protein was produced in Pichia pastoris. Physicochemical conditions and ligandation were intensively screened to obtain the best NMR spectra quality. This ns-LTP2 is a 67-residue globular protein with a diameter of about 30 A. The structure is composed of five helices forming a right superhelix. The protein presents an inner cavity, which has been measured at 341 A(3). All of the helices display hydrophobic side chains oriented toward the cavity. The phospholipid is found in this cavity. Its fatty acid chain is completely inserted in the protein, the l-alpha-palmitoylphosphatidylglycerol glycerol moiety being located on a positively charged pocket on the surface of the protein. The superhelix structure of the protein is coiled around the fatty acid chain. The overall structure shows similarities with ns-LTP1. Nevertheless, large three-dimensional structural discrepancies are observed for the H3 and H4 alpha-helices, the C-terminal region, and the last turn of the H2 helix. The lipid is orthogonal to the orientation observed in ns-LTP1. The volume of the hydrophobic cavity appears to be in the same range as the one of ns-LTP1, despite the fact that ns-LTP2 is shorter by 24 residues.

Refined solution structure of a liganded type 2 wheat nonspecific lipid transfer protein.,Pons JL, de Lamotte F, Gautier MF, Delsuc MA J Biol Chem. 2003 Apr 18;278(16):14249-56. Epub 2003 Jan 13. PMID:12525478^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Douliez JP, Pato C, Rabesona H, Molle D, Marion D. Disulfide bond assignment, lipid transfer activity and secondary structure of a 7-kDa plant lipid transfer protein, LTP2. Eur J Biochem. 2001 Mar;268(5):1400-3. PMID:11231292
↑ Pons JL, de Lamotte F, Gautier MF, Delsuc MA. Refined solution structure of a liganded type 2 wheat nonspecific lipid transfer protein. J Biol Chem. 2003 Apr 18;278(16):14249-56. Epub 2003 Jan 13. PMID:12525478 doi:10.1074/jbc.M211683200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n89"

@@ Line 1: / Line 1: @@
 ==Solution structure of a liganded type 2 wheat non-specific Lipid Transfer Protein==
-<StructureSection load='1n89' size='340' side='right' caption='[[1n89]], [[NMR_Ensembles_of_Models | 9 NMR models]]' scene=''>
+<StructureSection load='1n89' size='340' side='right'caption='[[1n89]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1n89]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Durum_wheat Durum wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N89 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N89 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1n89]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_turgidum_subsp._durum Triticum turgidum subsp. durum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N89 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGM:1-MYRISTOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]'>PGM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 9 models</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n89 OCA], [http://pdbe.org/1n89 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n89 RCSB], [http://www.ebi.ac.uk/pdbsum/1n89 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n89 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGM:1-MYRISTOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]'>PGM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n89 OCA], [https://pdbe.org/1n89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n89 RCSB], [https://www.ebi.ac.uk/pdbsum/1n89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n89 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NLT2G_WHEAT NLT2G_WHEAT]] Transfer lipids across membranes. May play a role in plant defense or in the biosynthesis of cuticle layers.<ref>PMID:11231292</ref>
+[https://www.uniprot.org/uniprot/NLT2G_WHEAT NLT2G_WHEAT] Transfer lipids across membranes. May play a role in plant defense or in the biosynthesis of cuticle layers.<ref>PMID:11231292</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n89_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n89_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Durum wheat]]
+[[Category: Large Structures]]
-[[Category: Delsuc, M A]]
+[[Category: Triticum turgidum subsp. durum]]
-[[Category: Gautier, M F]]
+[[Category: Delsuc MA]]
-[[Category: Lamotte, F de]]
+[[Category: Gautier MF]]
-[[Category: Pons, J L]]
+[[Category: Pons JL]]
-[[Category: Lipid transfer protein]]
+[[Category: De Lamotte F]]
-[[Category: Lipid transport]]

1n89

From Proteopedia

Current revision

Solution structure of a liganded type 2 wheat non-specific Lipid Transfer Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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