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1zhf

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[[Image:1zhf.gif|left|200px]]
 
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{{Structure
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==Crystal structure of selenomethionine substituted isoflavanone 4'-O-methyltransferase==
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|PDB= 1zhf |SIZE=350|CAPTION= <scene name='initialview01'>1zhf</scene>, resolution 2.50&Aring;
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<StructureSection load='1zhf' size='340' side='right'caption='[[1zhf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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<table><tr><td colspan='2'>[[1zhf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHF FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhf OCA], [https://pdbe.org/1zhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhf RCSB], [https://www.ebi.ac.uk/pdbsum/1zhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhf ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1zg3|1ZG3]], [[1zga|1ZGA]], [[1zgj|1ZGJ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhf OCA], [http://www.ebi.ac.uk/pdbsum/1zhf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zhf RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/I4OMT_MEDTR I4OMT_MEDTR]
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== Evolutionary Conservation ==
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'''Crystal structure of selenomethionine substituted isoflavanone 4'-O-methyltransferase'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/1zhf_consurf.spt"</scriptWhenChecked>
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In leguminous plants such as pea (Pisum sativum), alfalfa (Medicago sativa), barrel medic (Medicago truncatula), and chickpea (Cicer arietinum), 4'-O-methylation of isoflavonoid natural products occurs early in the biosynthesis of defense chemicals known as phytoalexins. However, among these four species, only pea catalyzes 3-O-methylation that converts the pterocarpanoid isoflavonoid 6a-hydroxymaackiain to pisatin. In pea, pisatin is important for chemical resistance to the pathogenic fungus Nectria hematococca. While barrel medic does not biosynthesize 6a-hydroxymaackiain, when cell suspension cultures are fed 6a-hydroxymaackiain, they accumulate pisatin. In vitro, hydroxyisoflavanone 4'-O-methyltransferase (HI4'OMT) from barrel medic exhibits nearly identical steady state kinetic parameters for the 4'-O-methylation of the isoflavonoid intermediate 2,7,4'-trihydroxyisoflavanone and for the 3-O-methylation of the 6a-hydroxymaackiain isoflavonoid-derived pterocarpanoid intermediate found in pea. Protein x-ray crystal structures of HI4'OMT substrate complexes revealed identically bound conformations for the 2S,3R-stereoisomer of 2,7,4'-trihydroxyisoflavanone and the 6aR,11aR-stereoisomer of 6a-hydroxymaackiain. These results suggest how similar conformations intrinsic to seemingly distinct chemical substrates allowed leguminous plants to use homologous enzymes for two different biosynthetic reactions. The three-dimensional similarity of natural small molecules represents one explanation for how plants may rapidly recruit enzymes for new biosynthetic reactions in response to changing physiological and ecological pressures.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1ZHF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHF OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zhf ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses., Liu CJ, Deavours BE, Richard SB, Ferrer JL, Blount JW, Huhman D, Dixon RA, Noel JP, Plant Cell. 2006 Dec;18(12):3656-69. Epub 2006 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17172354 17172354]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Medicago truncatula]]
[[Category: Medicago truncatula]]
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[[Category: Single protein]]
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[[Category: Deavours BE]]
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[[Category: Deavours, B E.]]
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[[Category: Dixon RA]]
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[[Category: Dixon, R A.]]
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[[Category: Ferrer J-L]]
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[[Category: Ferrer, J L.]]
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[[Category: Liu C-J]]
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[[Category: Liu, C J.]]
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[[Category: Noel JP]]
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[[Category: Noel, J P.]]
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[[Category: Richard S]]
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[[Category: Richard, S.]]
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[[Category: isoflavanone 4'-o-methyltransferase,rossman fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:36:34 2008''
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Current revision

Crystal structure of selenomethionine substituted isoflavanone 4'-O-methyltransferase

PDB ID 1zhf

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