6bff
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6bff is ON HOLD Authors: Smith, C.A., Vakulenko, S.B. Description: Structure of the aminoglycoside acetyltransferase AAC(6')-Im [[Category: Unrelea...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the aminoglycoside acetyltransferase AAC(6')-Im== | |
| + | <StructureSection load='6bff' size='340' side='right'caption='[[6bff]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6bff]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BFF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bff OCA], [https://pdbe.org/6bff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bff RCSB], [https://www.ebi.ac.uk/pdbsum/6bff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bff ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q93ET8_ECOLX Q93ET8_ECOLX] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aminoglycoside 6'-acetyltransferase-Im (AAC(6')-Im) is the closest monofunctional homolog of the AAC(6')-Ie acetyltransferase of the bifunctional enzyme AAC(6')-Ie/APH(2")-Ia. The AAC(6')-Im acetyltransferase confers 4- to 64-fold higher MICs to 4,6-disubstituted aminoglycosides and the 4,5-disubstituted aminoglycoside neomycin than AAC(6')-Ie, yet unlike AAC(6')-Ie, the AAC(6')-Im enzyme does not confer resistance to the atypical aminoglycoside fortimicin. The structure of the kanamycin A complex of AAC(6')-Im shows that the substrate binds in a shallow positively-charged pocket, with the N6' amino group positioned appropriately for an efficient nucleophilic attack on an acetyl-CoA cofactor. The AAC(6')-Ie enzyme binds kanamycin A in a sufficiently different manner to position the N6' group less efficiently, thereby reducing the activity of this enzyme towards the 4,6-disubstituted aminoglycosides. Conversely, docking studies with fortimicin in both acetyltransferases suggest that the atypical aminoglycoside might bind less productively in AAC(6')-Im, thus explaining the lack of resistance to this molecule. | ||
| - | + | Aminoglycoside resistance profile and structural architecture of the aminoglycoside acetyltransferase AAC(6')-Im.,Smith CA, Bhattacharya M, Toth M, Stewart NK, Vakulenko SB Microb Cell. 2017 Nov 9;4(12):402-410. doi: 10.15698/mic2017.12.602. PMID:29234669<ref>PMID:29234669</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6bff" style="background-color:#fffaf0;"></div> |
| - | [[Category: Smith | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Smith CA]] | ||
| + | [[Category: Vakulenko SB]] | ||
Current revision
Structure of the aminoglycoside acetyltransferase AAC(6')-Im
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