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| | ==Crystal structure of Ctf19-Mcm21 kinetochore assembly bound with Ctf19-Mcm21 binding motif of central kinetochore subunit Okp1== | | ==Crystal structure of Ctf19-Mcm21 kinetochore assembly bound with Ctf19-Mcm21 binding motif of central kinetochore subunit Okp1== |
| - | <StructureSection load='5mu3' size='340' side='right' caption='[[5mu3]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='5mu3' size='340' side='right'caption='[[5mu3]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mu3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MU3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mu3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MU3 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zxu|3zxu]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu3 OCA], [http://pdbe.org/5mu3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mu3 RCSB], [http://www.ebi.ac.uk/pdbsum/5mu3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu3 OCA], [https://pdbe.org/5mu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mu3 RCSB], [https://www.ebi.ac.uk/pdbsum/5mu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CTF19_KLULA CTF19_KLULA]] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation (By similarity). [[http://www.uniprot.org/uniprot/MCM21_KLULA MCM21_KLULA]] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation (By similarity). | + | [https://www.uniprot.org/uniprot/CENPO_KLULA CENPO_KLULA] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis (By similarity). Component of the inner kinetochore COMA subcomplex, which connects centromere-associated proteins and the outer kinetochore (PubMed:29046335). COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly (By similarity).[UniProtKB:Q06675]<ref>PMID:29046335</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Schmitzberger, F]] | + | [[Category: Kluyveromyces lactis NRRL Y-1140]] |
| - | [[Category: Cell cycle]] | + | [[Category: Large Structures]] |
| - | [[Category: Centromere]] | + | [[Category: Schmitzberger F]] |
| - | [[Category: Chromosome segregation]]
| + | |
| - | [[Category: Kinetochore]]
| + | |
| - | [[Category: Rwd domain]]
| + | |
| Structural highlights
Function
CENPO_KLULA Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis (By similarity). Component of the inner kinetochore COMA subcomplex, which connects centromere-associated proteins and the outer kinetochore (PubMed:29046335). COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly (By similarity).[UniProtKB:Q06675][1]
Publication Abstract from PubMed
Kinetochores are dynamic cellular structures that connect chromosomes to microtubules. They form from multi-protein assemblies that are evolutionarily conserved between yeasts and humans. One of these assemblies-COMA-consists of subunits Ame1CENP-U, Ctf19CENP-P, Mcm21CENP-O and Okp1CENP-Q A description of COMA molecular organization has so far been missing. We defined the subunit topology of COMA, bound with inner kinetochore proteins Nkp1 and Nkp2, from the yeast Kluyveromyces lactis, with nanoflow electrospray ionization mass spectrometry, and mapped intermolecular contacts with hydrogen-deuterium exchange coupled to mass spectrometry. Our data suggest that the essential Okp1 subunit is a multi-segmented nexus with distinct binding sites for Ame1, Nkp1-Nkp2 and Ctf19-Mcm21. Our crystal structure of the Ctf19-Mcm21 RWD domains bound with Okp1 shows the molecular contacts of this important inner kinetochore joint. The Ctf19-Mcm21 binding motif in Okp1 configures a branch of mitotic inner kinetochores, by tethering Ctf19-Mcm21 and Chl4CENP-N-Iml3CENP-L Absence of this motif results in dependence on the mitotic checkpoint for viability.
Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions.,Schmitzberger F, Richter MM, Gordiyenko Y, Robinson CV, Dadlez M, Westermann S EMBO J. 2017 Oct 18. pii: e201796636. doi: 10.15252/embj.201796636. PMID:29046335[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schmitzberger F, Richter MM, Gordiyenko Y, Robinson CV, Dadlez M, Westermann S. Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions. EMBO J. 2017 Oct 18. pii: e201796636. doi: 10.15252/embj.201796636. PMID:29046335 doi:http://dx.doi.org/10.15252/embj.201796636
- ↑ Schmitzberger F, Richter MM, Gordiyenko Y, Robinson CV, Dadlez M, Westermann S. Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions. EMBO J. 2017 Oct 18. pii: e201796636. doi: 10.15252/embj.201796636. PMID:29046335 doi:http://dx.doi.org/10.15252/embj.201796636
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