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| | ==Molecular insights into the specificity of exfoliative toxins from Staphylococcus aureus== | | ==Molecular insights into the specificity of exfoliative toxins from Staphylococcus aureus== |
| - | <StructureSection load='5c2z' size='340' side='right' caption='[[5c2z]], [[Resolution|resolution]] 1.96Å' scene=''> | + | <StructureSection load='5c2z' size='340' side='right'caption='[[5c2z]], [[Resolution|resolution]] 1.96Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5c2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C2Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5c2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C2Z FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">etD2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9553Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c2z OCA], [http://pdbe.org/5c2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c2z RCSB], [http://www.ebi.ac.uk/pdbsum/5c2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c2z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c2z OCA], [https://pdbe.org/5c2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c2z RCSB], [https://www.ebi.ac.uk/pdbsum/5c2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c2z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/L0RUV7_STAAU L0RUV7_STAAU] |
| - | Exfoliative toxins are serine proteases secreted by Staphylococcus aureus that are associated with toxin-mediated staphylococcal syndromes. To date, four different serotypes of exfoliative toxins have been identified and 3 of them (ETA, ETB, and ETD) are linked to human infection. Among these toxins, only the ETD structure remained unknown, limiting our understanding of the structural determinants for the functional differentiation between these toxins. We recently identified an ETD-like protein associated to S. aureus strains involved in mild mastitis in sheep. The crystal structure of this ETD-like protein was determined at 1.95 A resolution and the structural analysis provide insights into the oligomerization, stability and specificity and enabled a comprehensive structural comparison with ETA and ETB. Despite the highly conserved molecular architecture, significant differences in the composition of the loops and in both the N- and C-terminal alpha-helices seem to define ETD-like specificity. Molecular dynamics simulations indicate that these regions defining ET specificity present different degrees of flexibility and may undergo conformational changes upon substrate recognition and binding. DLS and AUC experiments indicated that the ETD-like is monomeric in solution whereas it is present as a dimer in the asymmetric unit indicating that oligomerization is not related to functional differentiation among these toxins. Differential scanning calorimetry and circular dichroism assays demonstrated an endothermic transition centered at 52 degrees C, and an exothermic aggregation in temperatures up to 64 degrees C. All these together provide insights about the mode of action of a toxin often secreted in syndromes that are not associated with either ETA or ETB.
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| - | Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins.,Mariutti RB, Souza TA, Ullah A, Caruso IP, de Moraes FR, Zanphorlin LM, Tartaglia NR, Seyffert N, Azevedo VA, Le Loir Y, Murakami MT, Arni RK Biochem Biophys Res Commun. 2015 Nov 6;467(1):171-7. doi:, 10.1016/j.bbrc.2015.08.083. Epub 2015 Aug 20. PMID:26299923<ref>PMID:26299923</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5c2z" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arni, R K]] | + | [[Category: Large Structures]] |
| - | [[Category: Mariutti, R B]]
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| - | [[Category: Murakami, M T]]
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| - | [[Category: Souza, T A.C B]]
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| - | [[Category: Ullah, A]]
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| - | [[Category: Zanphorlin, L M]]
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| - | [[Category: Esfoliative toxin]]
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| - | [[Category: Etd]]
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| | [[Category: Staphylococcus aureus]] | | [[Category: Staphylococcus aureus]] |
| - | [[Category: Toxin]] | + | [[Category: Arni RK]] |
| | + | [[Category: Mariutti RB]] |
| | + | [[Category: Murakami MT]] |
| | + | [[Category: Souza TACB]] |
| | + | [[Category: Ullah A]] |
| | + | [[Category: Zanphorlin LM]] |
