5xz0
From Proteopedia
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==Staphylococcal Enterotoxin B (SEB) mutant S19 - N23A, Y90A, R110A and F177A== | ==Staphylococcal Enterotoxin B (SEB) mutant S19 - N23A, Y90A, R110A and F177A== | ||
| - | <StructureSection load='5xz0' size='340' side='right' caption='[[5xz0]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='5xz0' size='340' side='right'caption='[[5xz0]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5xz0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZ0 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5xz0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XZ0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.002Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xz0 OCA], [https://pdbe.org/5xz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xz0 RCSB], [https://www.ebi.ac.uk/pdbsum/5xz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xz0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H2WZB2_STAAC A0A0H2WZB2_STAAC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Four mutations (N23A, Y90A, R110A and F177A) were introduced into S19, a vaccine candidate for staphylococcal enterotoxin B (SEB), resulting in a lower binding affinity towards the T-cell receptor beta chain (TCB) and reducing its superantigen activity. The structure of S19 was solved and was superposed on the native or complex structure of SEB. In the superposition model, mutations that were introduced seemed to reduce the number of hydrogen bonds at the SEB-TCB interface. S19 also displayed an unexpected structural change around the flexible-loop region owing to the Y90A mutation. This local structural change provided evidence that the mutated form of S19 could have a lower affinity for major histocompatibility complex (MHC) class II than wild-type SEB. | ||
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| + | Structure of the staphylococcal enterotoxin B vaccine candidate S19 showing eliminated superantigen activity.,Jeong WH, Song DH, Hur GH, Jeong ST Acta Crystallogr F Struct Biol Commun. 2017 Nov 1;73(Pt 11):595-600. doi:, 10.1107/S2053230X17014844. Epub 2017 Oct 20. PMID:29095152<ref>PMID:29095152</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5xz0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Staphylococcus aureus subsp. aureus COL]] |
| - | [[Category: | + | [[Category: Hur GH]] |
| - | [[Category: | + | [[Category: Jeong ST]] |
| - | [[Category: | + | [[Category: Jeong WH]] |
| - | [[Category: | + | [[Category: Song DH]] |
| - | + | ||
| - | + | ||
Current revision
Staphylococcal Enterotoxin B (SEB) mutant S19 - N23A, Y90A, R110A and F177A
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