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| - | [[Image:1zo3.gif|left|200px]] | |
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| - | {{Structure
| + | ==The P-site and P/E-site tRNA structures fitted to P/I site codon.== |
| - | |PDB= 1zo3 |SIZE=350|CAPTION= <scene name='initialview01'>1zo3</scene>
| + | <SX load='1zo3' size='340' side='right' viewer='molstar' caption='[[1zo3]], [[Resolution|resolution]] 13.80Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[1zo3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZO3 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.8Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zo3 OCA], [https://pdbe.org/1zo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zo3 RCSB], [https://www.ebi.ac.uk/pdbsum/1zo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zo3 ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=[[1zo1|1ZO1]]
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zo3 OCA], [http://www.ebi.ac.uk/pdbsum/1zo3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zo3 RCSB]</span>
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| - | }}
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| | | | |
| - | '''The P-site and P/E-site tRNA structures fitted to P/I site codon.'''
| + | ==See Also== |
| - | | + | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </SX> |
| - | The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.
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| - | ==About this Structure==
| + | |
| - | 1ZO3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15935757 15935757]
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| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| - | [[Category: Protein complex]] | + | [[Category: Large Structures]] |
| - | [[Category: Allen, G S.]] | + | [[Category: Allen GS]] |
| - | [[Category: Ehrenberg, M.]] | + | [[Category: Ehrenberg M]] |
| - | [[Category: Frank, J.]] | + | [[Category: Frank J]] |
| - | [[Category: Gursky, R.]] | + | [[Category: Gursky R]] |
| - | [[Category: Zavialov, A.]] | + | [[Category: Zavialov A]] |
| - | [[Category: cryo-eletron microscopy]]
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| - | [[Category: e. coli]]
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| - | [[Category: initiation factor]]
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| - | [[Category: initiation of protein synthesis]]
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| - | [[Category: ribosome]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:39:24 2008''
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