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| | ==Crystal structure of a putative neuraminidase from Pseudomonas aeruginosa== | | ==Crystal structure of a putative neuraminidase from Pseudomonas aeruginosa== |
| - | <StructureSection load='3h6j' size='340' side='right' caption='[[3h6j]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='3h6j' size='340' side='right'caption='[[3h6j]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3h6j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H6J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H6J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3h6j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H6J FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3h71|3h71]], [[3h72|3h72]], [[3h73|3h73]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA2794 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h6j OCA], [https://pdbe.org/3h6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h6j RCSB], [https://www.ebi.ac.uk/pdbsum/3h6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h6j ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h6j OCA], [http://pdbe.org/3h6j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3h6j RCSB], [http://www.ebi.ac.uk/pdbsum/3h6j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3h6j ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q9L6G4_PSEAI Q9L6G4_PSEAI] |
| - | Currently there is pressing need to develop novel therapeutic agents for the treatment of infections by the human respiratory pathogens Pseudomonas aeruginosa and Streptococcus pneumoniae. The neuraminidases of these pathogens are important for host colonization in animal models of infection and are attractive targets for drug discovery. To aid in the development of inhibitors against these neuraminidases, we have determined the crystal structures of the P. aeruginosa enzyme NanPs and S. pneumoniae enzyme NanA at 1.6 and 1.7A resolution, respectively. In situ proteolysis with trypsin was essential for the crystallization of our recombinant NanA. The active site regions of the two enzymes are strikingly different. NanA contains a deep pocket that is similar to that in canonical neuraminidases, while the NanPs active site is much more open. The comparative studies suggest that NanPs may not be a classical neuraminidase, and may have distinct natural substrates and physiological functions. This work represents an important step in the development of drugs to prevent respiratory tract colonization by these two pathogens.
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| - | Crystal structures of respiratory pathogen neuraminidases.,Hsiao YS, Parker D, Ratner AJ, Prince A, Tong L Biochem Biophys Res Commun. 2009 Mar 13;380(3):467-71. Epub 2009 Jan 23. PMID:19284989<ref>PMID:19284989</ref>
| + | ==See Also== |
| - | | + | *[[Neuraminidase 3D structures|Neuraminidase 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3h6j" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hsiao, Y S]] | + | [[Category: Large Structures]] |
| - | [[Category: Tong, L]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Cell wall]] | + | [[Category: Hsiao Y-S]] |
| - | [[Category: Glycosidase]]
| + | [[Category: Tong L]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Peptidoglycan-anchor]]
| + | |
| - | [[Category: Secreted]] | + | |
| - | [[Category: Six-bladed beta-propeller]]
| + | |
