1zp7

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[[Image:1zp7.gif|left|200px]]
 
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{{Structure
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==The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.==
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|PDB= 1zp7 |SIZE=350|CAPTION= <scene name='initialview01'>1zp7</scene>, resolution 2.25&Aring;
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<StructureSection load='1zp7' size='340' side='right'caption='[[1zp7]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1zp7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZP7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zp7 OCA], [https://pdbe.org/1zp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zp7 RCSB], [https://www.ebi.ac.uk/pdbsum/1zp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zp7 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zp7 OCA], [http://www.ebi.ac.uk/pdbsum/1zp7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zp7 RCSB]</span>
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[https://www.uniprot.org/uniprot/RECU_BACSU RECU_BACSU] Has at least 2 separable functions; Holliday junction resolution with generation of monomeric chromosomes, and modulation of RecA activity. Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation. Partially inhibits the hydrolysis of dATP or rATP by RecA. Holliday junction resolution is stimulated by RuvB.<ref>PMID:14701911</ref> <ref>PMID:16020779</ref> <ref>PMID:16154091</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zp/1zp7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zp7 ConSurf].
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<div style="clear:both"></div>
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'''The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.'''
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==See Also==
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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== References ==
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==Overview==
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<references/>
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We have determined the structure of the enzyme RecU from Bacillus subtilis, that is the general Holliday junction resolving enzyme in Gram-positive bacteria. The enzyme fold reveals a striking similarity to a class of resolvase enzymes found in archaeal sources and members of the type II restriction endonuclease family to which they are related. The structure confirms the presence of active sites formed around clusters of acidic residues that we have also shown to bind divalent cations. Mutagenesis data presented here support the key role of certain residues. The RecU structure suggests a basis for Holliday junction selectivity and suggests how sequence-specific cleavage might be achieved. Models for a resolvase-DNA complex address how the enzyme might organize junctions into an approximately 4-fold symmetric form.
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__TOC__
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</StructureSection>
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==About this Structure==
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1ZP7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZP7 OCA].
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==Reference==
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The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage., McGregor N, Ayora S, Sedelnikova S, Carrasco B, Alonso JC, Thaw P, Rafferty J, Structure. 2005 Sep;13(9):1341-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16154091 16154091]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alonso, J C.]]
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[[Category: Alonso JC]]
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[[Category: Ayora, S.]]
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[[Category: Ayora S]]
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[[Category: Carrasco, B.]]
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[[Category: Carrasco B]]
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[[Category: McGregor, N.]]
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[[Category: McGregor N]]
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[[Category: Rafferty, J.]]
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[[Category: Rafferty J]]
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[[Category: Sedelnikova, S.]]
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[[Category: Sedelnikova S]]
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[[Category: Thaw, P.]]
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[[Category: Thaw P]]
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[[Category: recombination,dna-binding protein,resolvase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:39:47 2008''
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Current revision

The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.

PDB ID 1zp7

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