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| | ==Crystal Structure of single chain PvuII== | | ==Crystal Structure of single chain PvuII== |
| - | <StructureSection load='3ksk' size='340' side='right' caption='[[3ksk]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='3ksk' size='340' side='right'caption='[[3ksk]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ksk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"proteus_hauseri"_kauffmann_1954 "proteus hauseri" kauffmann 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KSK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ksk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_hauseri Proteus hauseri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KSK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pvu|1pvu]], [[1pvi|1pvi]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pvuII, pvuIIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=183417 "Proteus hauseri" Kauffmann 1954])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ksk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ksk OCA], [https://pdbe.org/3ksk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ksk RCSB], [https://www.ebi.ac.uk/pdbsum/3ksk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ksk ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr> | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ksk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ksk OCA], [http://pdbe.org/3ksk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ksk RCSB], [http://www.ebi.ac.uk/pdbsum/3ksk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ksk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU]] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3. | + | [https://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The restriction endonuclease PvuII from Proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scPvuII by tandemly joining the two subunits through the peptide linker Gly-Ser-Gly-Gly. scPvuII, which is suitable for the development of programmed restriction endonucleases for highly specific DNA cleavage, was purified and crystallized. The crystals diffract to a resolution of 2.35 A and belong to space group P4(2), with unit-cell parameters a = b = 101.92, c = 100.28 A and two molecules per asymmetric unit. Phasing was successfully performed by molecular replacement.
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| - | Purification, crystallization, X-ray diffraction analysis and phasing of an engineered single-chain PvuII restriction endonuclease.,Meramveliotaki C, Kotsifaki D, Androulaki M, Hountas A, Eliopoulos E, Kokkinidis M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt, 10):836-8. Epub 2007 Sep 19. PMID:17909283<ref>PMID:17909283</ref>
| + | ==See Also== |
| - | | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3ksk" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Proteus hauseri kauffmann 1954]] | + | [[Category: Large Structures]] |
| - | [[Category: Type II site-specific deoxyribonuclease]] | + | [[Category: Proteus hauseri]] |
| - | [[Category: Eliopoulos, E]] | + | [[Category: Eliopoulos E]] |
| - | [[Category: Hountas, A]] | + | [[Category: Hountas A]] |
| - | [[Category: Kokkinidis, M]] | + | [[Category: Kokkinidis M]] |
| - | [[Category: Meramveliotaki, C]] | + | [[Category: Meramveliotaki C]] |
| - | [[Category: Dna-binding protein]]
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| - | [[Category: Endonuclease]]
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| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Magnesium]]
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| - | [[Category: Metal-binding]]
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| - | [[Category: Nuclease]]
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| - | [[Category: Restriction system]]
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| - | [[Category: Single chain restriction endonuclease]]
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