5cyv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:08, 13 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
==Crystal structure of CouR from Rhodococcus jostii RHA1 bound to p-coumaroyl-CoA==
==Crystal structure of CouR from Rhodococcus jostii RHA1 bound to p-coumaroyl-CoA==
-
<StructureSection load='5cyv' size='340' side='right' caption='[[5cyv]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
+
<StructureSection load='5cyv' size='340' side='right'caption='[[5cyv]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5cyv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhojr Rhojr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CYV FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5cyv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_jostii_RHA1 Rhodococcus jostii RHA1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CYV FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=WCA:P-COUMAROYL-COA'>WCA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3fm5|3fm5]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=WCA:P-COUMAROYL-COA'>WCA</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RHA1_ro05125 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=101510 RHOJR]), CouR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=101510 RHOJR])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cyv OCA], [https://pdbe.org/5cyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cyv RCSB], [https://www.ebi.ac.uk/pdbsum/5cyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cyv ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cyv OCA], [http://pdbe.org/5cyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cyv RCSB], [http://www.ebi.ac.uk/pdbsum/5cyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cyv ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q0S6D0_RHOJR Q0S6D0_RHOJR]
-
CouR, a MarR-type transcriptional repressor, regulates the cou genes, encoding p-hydroxycinnamate catabolism in the soil bacterium Rhodococcus jostii RHA1. The CouR dimer bound two molecules of the catabolite p-coumaroyl-CoA (Kd = 11 +/- 1 muM). The presence of p-coumaroyl-CoA, but neither p-coumarate nor CoASH, abrogated CouR's binding to its operator DNA in vitro. The crystal structures of ligand-free CouR and its p-coumaroyl-CoA-bound form showed no significant conformational differences, in contrast to other MarR regulators. The CouR-p-coumaroyl-CoA structure revealed two ligand molecules bound to the CouR dimer with their phenolic moieties occupying equivalent hydrophobic pockets in each protomer and their CoA moieties adopting non-equivalent positions to mask the regulator's predicted DNA-binding surface. More specifically, the CoA phosphates formed salt bridges with predicted DNA-binding residues Arg36 and Arg38, changing the overall charge of the DNA-binding surface. The substitution of either arginine with alanine completely abrogated the ability of CouR to bind DNA. By contrast, the R36A/R38A double variant retained a relatively high affinity for p-coumaroyl-CoA (Kd = 89 +/- 6 muM). Together, our data point to a novel mechanism of action in which the ligand abrogates the repressor's ability to bind DNA by steric occlusion of key DNA-binding residues and charge repulsion of the DNA backbone.
+
-
The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism.,Otani H, Stogios PJ, Xu X, Nocek B, Li SN, Savchenko A, Eltis LD Nucleic Acids Res. 2016 Jan 29;44(2):595-607. doi: 10.1093/nar/gkv955. Epub 2015 , Sep 22. PMID:26400178<ref>PMID:26400178</ref>
+
==See Also==
-
 
+
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5cyv" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Rhojr]]
+
[[Category: Large Structures]]
-
[[Category: Dong, A]]
+
[[Category: Rhodococcus jostii RHA1]]
-
[[Category: Joachimiak, A]]
+
[[Category: Dong A]]
-
[[Category: Structural genomic]]
+
[[Category: Joachimiak A]]
-
[[Category: Savchenko, A]]
+
[[Category: Savchenko A]]
-
[[Category: Stogios, P J]]
+
[[Category: Stogios PJ]]
-
[[Category: Xu, X]]
+
[[Category: Xu X]]
-
[[Category: Marr]]
+
-
[[Category: Mcsg]]
+
-
[[Category: P-hydroxycinnamate metabolism]]
+
-
[[Category: Pf04017]]
+
-
[[Category: PSI, Protein structure initiative]]
+
-
[[Category: Psi-biology]]
+
-
[[Category: Transcription regulator]]
+
-
[[Category: Transcriptional regulator]]
+

Current revision

Crystal structure of CouR from Rhodococcus jostii RHA1 bound to p-coumaroyl-CoA

PDB ID 5cyv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5cyv"
Personal tools