|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of the C-terminal domain of TnsE at 2.85 resolution== | | ==Structure of the C-terminal domain of TnsE at 2.85 resolution== |
| - | <StructureSection load='5d17' size='340' side='right' caption='[[5d17]], [[Resolution|resolution]] 2.85Å' scene=''> | + | <StructureSection load='5d17' size='340' side='right'caption='[[5d17]], [[Resolution|resolution]] 2.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5d17]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D17 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5d17]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D17 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d16|5d16]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tnsE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d17 OCA], [https://pdbe.org/5d17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d17 RCSB], [https://www.ebi.ac.uk/pdbsum/5d17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d17 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d17 OCA], [http://pdbe.org/5d17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d17 RCSB], [http://www.ebi.ac.uk/pdbsum/5d17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d17 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TNSE_ECOLX TNSE_ECOLX]] TnsABC + TnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas TnsABC + TnsE promote low-frequency insertion into many different sites. | + | [https://www.uniprot.org/uniprot/TNSE_ECOLX TNSE_ECOLX] TnsABC + TnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas TnsABC + TnsE promote low-frequency insertion into many different sites. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 19: |
| | </div> | | </div> |
| | <div class="pdbe-citations 5d17" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5d17" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Transposase 3D structures|Transposase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Caron, J J]] | + | [[Category: Large Structures]] |
| - | [[Category: Guarne, A]] | + | [[Category: Caron JJ]] |
| - | [[Category: Conformational toggle]] | + | [[Category: Guarne A]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Tn7]]
| + | |
| - | [[Category: Transposition]]
| + | |
| Structural highlights
Function
TNSE_ECOLX TnsABC + TnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas TnsABC + TnsE promote low-frequency insertion into many different sites.
Publication Abstract from PubMed
The bacterial transposon Tn7 facilitates horizontal transfer by directing transposition into actively replicating DNA with the element-encoded protein TnsE. Structural analysis of the C-terminal domain of wild-type TnsE identified a novel protein fold including a central V-shaped loop that toggles between two distinct conformations. The structure of a robust TnsE gain-of-activity variant has this loop locked in a single conformation, suggesting that conformational flexibility regulates TnsE activity. Structure-based analysis of a series of TnsE mutants relates transposition activity to DNA binding stability. Wild-type TnsE appears to naturally form an unstable complex with a target DNA, whereas mutant combinations required for large changes in transposition frequency and targeting stabilized this interaction. Collectively, our work unveils a unique structural proofreading mechanism where toggling between two conformations regulates target commitment by limiting the stability of target DNA engagement until an appropriate insertion site is identified.
Conformational toggling controls target site choice for the heteromeric transposase element Tn7.,Shi Q, Straus MR, Caron JJ, Wang H, Chung YS, Guarne A, Peters JE Nucleic Acids Res. 2015 Sep 17. pii: gkv913. PMID:26384427[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Shi Q, Straus MR, Caron JJ, Wang H, Chung YS, Guarne A, Peters JE. Conformational toggling controls target site choice for the heteromeric transposase element Tn7. Nucleic Acids Res. 2015 Sep 17. pii: gkv913. PMID:26384427 doi:http://dx.doi.org/10.1093/nar/gkv913
|
