1zzc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:07, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1zzc.gif|left|200px]]
 
-
{{Structure
+
==Crystal Structure of CoII HppE in Complex with Tris Buffer==
-
|PDB= 1zzc |SIZE=350|CAPTION= <scene name='initialview01'>1zzc</scene>, resolution 1.80&Aring;
+
<StructureSection load='1zzc' size='340' side='right'caption='[[1zzc]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
+
<table><tr><td colspan='2'>[[1zzc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZZC FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
|GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzc OCA], [https://pdbe.org/1zzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zzc RCSB], [https://www.ebi.ac.uk/pdbsum/1zzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zzc ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1zz6|1ZZ6]], [[1zz7|1ZZ7]], [[1zz8|1ZZ8]], [[1zz9|1ZZ9]], [[1zzb|1ZZB]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzc OCA], [http://www.ebi.ac.uk/pdbsum/1zzc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zzc RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/HPPE_STRWE HPPE_STRWE] Non-heme-dependent dioxygenase that catalyzes the oxidative epoxidation of (S)-2-hydroxypropylphosphonate into (1R,2S)-epoxypropylphosphonate, the final step in the biosynthesis of fosfomycin antibiotic.<ref>PMID:16015285</ref> <ref>PMID:16186494</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/1zzc_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zzc ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal Structure of CoII HppE in Complex with Tris Buffer'''
+
==See Also==
-
 
+
*[[Epoxidase 3D structures|Epoxidase 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
The biosynthetic pathway of the clinically important antibiotic fosfomycin uses enzymes that catalyse reactions without precedent in biology. Among these is hydroxypropylphosphonic acid epoxidase, which represents a new subfamily of non-haem mononuclear iron enzymes. Here we present six X-ray structures of this enzyme: the apoenzyme at 2.0 A resolution; a native Fe(II)-bound form at 2.4 A resolution; a tris(hydroxymethyl)aminomethane-Co(II)-enzyme complex structure at 1.8 A resolution; a substrate-Co(II)-enzyme complex structure at 2.5 A resolution; and two substrate-Fe(II)-enzyme complexes at 2.1 and 2.3 A resolution. These structural data lead us to suggest how this enzyme is able to recognize and respond to its substrate with a conformational change that protects the radical-based intermediates formed during catalysis. Comparisons with other family members suggest why substrate binding is able to prime iron for dioxygen binding in the absence of alpha-ketoglutarate (a co-substrate required by many mononuclear iron enzymes), and how the unique epoxidation reaction of hydroxypropylphosphonic acid epoxidase may occur.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1ZZC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZC OCA].
+
-
 
+
-
==Reference==
+
-
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16015285 16015285]
+
-
[[Category: Single protein]]
+
[[Category: Streptomyces wedmorensis]]
[[Category: Streptomyces wedmorensis]]
-
[[Category: Drennan, C L.]]
+
[[Category: Drennan CL]]
-
[[Category: Higgins, L J.]]
+
[[Category: Higgins LJ]]
-
[[Category: Liu, H W.]]
+
[[Category: Liu HW]]
-
[[Category: Liu, P.]]
+
[[Category: Liu P]]
-
[[Category: Yan, F.]]
+
[[Category: Yan F]]
-
[[Category: cupin]]
+
-
[[Category: holo-hppe]]
+
-
[[Category: mononuclear iron enzyme]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:43:42 2008''
+

Current revision

Crystal Structure of CoII HppE in Complex with Tris Buffer

PDB ID 1zzc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zzc"
Personal tools