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5ii7

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In-house sulfur-SAD structure of orthorhombic red abalone lysin at 1.66 A resolution

Structural highlights

5ii7 is a 1 chain structure with sequence from Haliotis rufescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.66Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ELYS_HALRU Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.

Publication Abstract from PubMed

Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.

Structural Basis of Egg Coat-Sperm Recognition at Fertilization.,Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L. Structural Basis of Egg Coat-Sperm Recognition at Fertilization. Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512 doi:http://dx.doi.org/10.1016/j.cell.2017.05.033

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ii7"

@@ Line 1: / Line 1: @@
 ==In-house sulfur-SAD structure of orthorhombic red abalone lysin at 1.66 A resolution==
-<StructureSection load='5ii7' size='340' side='right' caption='[[5ii7]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
+<StructureSection load='5ii7' size='340' side='right'caption='[[5ii7]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ii7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5II7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ii7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haliotis_rufescens Haliotis rufescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5II7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lis|1lis]], [[2lis|2lis]], [[1lyn|1lyn]], [[2lyn|2lyn]], [[5ii8|5ii8]], [[5ii9|5ii9]], [[5iia|5iia]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ii7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii7 OCA], [http://pdbe.org/5ii7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ii7 RCSB], [http://www.ebi.ac.uk/pdbsum/5ii7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ii7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii7 OCA], [https://pdbe.org/5ii7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ii7 RCSB], [https://www.ebi.ac.uk/pdbsum/5ii7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU]] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
+[https://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 5ii7" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysin 3D structures|Lysin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Al-Hosseini, H Sadat]]
+[[Category: Haliotis rufescens]]
-[[Category: Jovine, L]]
+[[Category: Large Structures]]
-[[Category: Nishimura, K]]
+[[Category: Jovine L]]
-[[Category: Raj, I]]
+[[Category: Nishimura K]]
-[[Category: Acrosomal protein]]
+[[Category: Raj I]]
-[[Category: Cell adhesion]]
+[[Category: Sadat Al-Hosseini H]]
-[[Category: Crystal dehydration]]
-[[Category: Egg coat penetration]]
-[[Category: Egg-binding protein]]
-[[Category: Egg-sperm interaction]]
-[[Category: Fertilization]]
-[[Category: Gamete recognition]]
-[[Category: S-sad]]
-[[Category: Sulfur-sad]]

5ii7

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Current revision

In-house sulfur-SAD structure of orthorhombic red abalone lysin at 1.66 A resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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